Long Noncoding RNA NEAT1 Suppresses Proliferation and Promotes Apoptosis of Glioma Cells Via Downregulating MiR-92b

• Published in: Cancer control 2020-01, Vol.27 (1), p.1073274819897977-1073274819897977
• Main Authors: Liu, Dongdong, Zou, Zheng, Li, Gen, Pan, Pengyu, Liang, Guobiao
• Format: Article
• Language: English
• Subjects: Apoptosis; Apoptosis - physiology; Astrocytes; Brain Neoplasms - genetics; Brain Neoplasms - metabolism; Brain Neoplasms - pathology; Cell growth; Cell proliferation; Cell Proliferation - physiology; Down-Regulation; Flow cytometry; Glioma; Glioma - genetics; Glioma - metabolism; Glioma - pathology; Glioma cells; Humans; Immunoprecipitation; MicroRNAs - genetics; MicroRNAs - metabolism; miRNA; Polymerase chain reaction; RNA, Long Noncoding - genetics; RNA, Long Noncoding - metabolism; Transcription; Transfection; Tumor suppressor genes
• ISSN: 1073-2748; 1526-2359; 1073-2748
• DOI: 10.1177/1073274819897977

Background: The mechanisms underlying the proliferation and apoptosis of glioma cells remain unelucidated. A recent study has revealed that microRNA-92b (miR-92b) inhibits apoptosis of glioma cells via downregulating DKK3. Notably, long noncoding RNA nuclear-enriched abundant transcript 1 (NEAT1) is predicted to have a possible interaction with miR-92b. Objective: This study aimed to identify whether NEAT1 affects glioma cell proliferation and apoptosis via regulating miR-92b. Methods: The expression of NEAT1 was compared between glioma tissues and adjacent tissues as well as between glioma cells and normal astrocytes using quantitative real-time polymerase chain reaction. Glioma cell proliferation was determined by using the 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, and glioma cell apoptosis was determined by using the flow cytometry. Results: The expression of NEAT1 was low in glioma tissues and cells compared to the normal ones. Overexpression of NEAT1 inhibited proliferation and promoted apoptosis of glioma cell lines (U-87 MG and U251). The interaction between NEAT1 and miR-92b was confirmed using RNA immunoprecipitation, RNA pull-down assay, and luciferase reporter assay. Importantly, the tumor suppressor function of overexpressing NEAT1 was achieved by downregulating miR-92b and subsequently upregulating DKK3. Conclusion: Our findings indicated that NEAT1 acts as a tumor suppressor in glioma cells, which provides a novel target in overcoming glioma growth.