Evaluation of surface roughness, wettability and adhesion of multispecies biofilm on 3D-printed resins for the base and teeth of complete dentures

• Published in: Journal of applied oral science 2024-01, Vol.32, p.e20230326-e20230326
• Main Authors: Poker, Beatriz de Camargo, Oliveira, Viviane de Cássia, Macedo, Ana Paula, Gonçalves, Mariane, Ramos, Ana Paula, Silva-Lovato, Cláudia Helena
• Format: Article
• Language: English
• Subjects: Acrylic resins; Acrylic Resins - chemistry; Analysis of Variance; Bacterial Adhesion; Biofilms; Candida albicans - physiology; Candidiasis; Colony Count, Microbial; Complete dentures; Computer-aided design; DENTISTRY, ORAL SURGERY & MEDICINE; Denture Bases - microbiology; Denture, Complete - microbiology; Linear Models; Materials Testing; Microscopy, Confocal; Microscopy, Electron, Scanning; Printing, Three-Dimensional; Reference Values; Reproducibility of Results; Staphylococcus aureus - physiology; Streptococcus mutans - physiology; Surface Properties; Three-dimensional printing; Wettability
• ISSN: 1678-7757; 1678-7765; 1678-7765
• DOI: 10.1590/1678-7757-2023-0326

This study evaluated the surface roughness, wettability and adhesion of multispecies biofilms (Candida albicans, Staphylococcus aureus and Streptococcus mutans) on 3D-printed resins for complete denture bases and teeth compared to conventional resins (heat-polymerized acrylic resin; artificial pre-fabricated teeth). Circular specimens (n=39; 6.0 mm Ø × 2.0 mm) of each group were subjected to roughness (n=30), wettability (n=30) and biofilm adhesion (n=9) tests. Three roughness measurements were taken by laser confocal microscopy and a mean value was calculated. Wettability was evaluated by the contact angle of sessile drop method, considering the mean of the three evaluations per specimen. In parallel, microorganism adhesion to resin surfaces was evaluated using a multispecies biofilm model. Microbial load was evaluated by determining the number of Colony Forming Units (CFU/mL) and by scanning electron microscopy (SEM). Data were subjected to the Wald test in a generalized linear model with multiple comparisons and Bonferroni adjustment, as well as two-way ANOVA (α=5%). The roughness of the conventional base resin (0.01±0.04) was lower than that of the conventional tooth (0.14±0.04) (p=0.023) and 3D-printed base (0.18±0.08) (p