RNA interference in cytochrome P450 monooxygenase (CYP) gene results in reduced insecticide resistance in Megalurothrips usitatus Bagnall

• Published in: Frontiers in physiology 2023-03, Vol.14, p.1130389-1130389
• Main Authors: Chen, Weiyi, Li, Zhaoyang, Zhou, Chenyan, Ali, Asad, Ali, Shaukat, Wu, Jianhui
• Format: Article
• Language: English
• Subjects: cytochrome P-450; insecticide resistance; Megalurothrips usitatus (Bagnall); Physiology; RNA interference; transcriptome
• ISSN: 1664-042X; 1664-042X
• DOI: 10.3389/fphys.2023.1130389

Genes of the cytochrome P450 (CYP450) superfamily are known to be involved in the evolution of insecticide resistance. In this study, the transcriptomes of two Bagnall (Thysanoptera: Thripidae) strains (resistant and susceptible) were screened for detoxification genes. encodes a protein composed of 504 amino acid residues with a relative molecular mass of 57.3 kDa. Multiple sequence alignment and phylogenetic analysis showed that is a member of the CYP450 family and has characteristics of the conserved CYP6 domain shared by typical CYP450 family members. RT-qPCR (real-time quantitative polymerase chain reaction) analysis showed that was upregulated in the acetamiprid-resistant strain compared with the susceptible strain ( < 0.05), and the relative expression level was significantly higher at 48 h after exposure than at 24 h after exposure. The interference efficiency of the injection method was higher than that of the membrane-feeding method. Silencing of through RNA interference significantly increased the sensitivity of to acetamiprid. Overall, this study revealed that plays a crucial role in the resistance of to acetamiprid. The findings will not only advance our understanding of the role of P450s in insecticide resistance but also provide a potential target for the sustainable control of destructive pests such as thrips.