Proteomic analysis illustrates the potential involvement of motor proteins in cleft palate development

Cleft palate (CP) is a congenital condition characterized by a complex etiology and limited diagnostic and therapeutic options. In this study, we delved into the molecular mechanisms associated with retinoic acid (RA)-induced CP in Kun Ming mice. Proteomic analysis of control and RA-induced CP sampl...

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Bibliographic Details
Published in:Scientific reports 2024-09, Vol.14 (1), p.21868-8, Article 21868
Main Authors: Huang, Zijian, Zhang, Chuzhao, Sun, Meng, Ma, Aiwei, Chen, Liyun, Jiang, Wenshi, Xu, Mengjing, Bai, Xujue, Zhou, Jianda, Zhang, Wancong, Tang, Shijie
Format: Article
Language:English
Subjects:
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631/45
Actin
Animal euthanasia
Animals
Birth defects
Cleft lip/palate
Cleft palate
Cleft Palate - genetics
Cleft Palate - metabolism
Cleft Palate - pathology
Cytoskeleton
Differentially expressed proteins
Disease Models, Animal
Down-regulation
Embryogenesis
Extracellular matrix
Female
Females
Gene Expression Regulation, Developmental
Humanities and Social Sciences
Investigations
Laboratory animals
Medical schools
Mice
Molecular modelling
Motor proteins
Motor task performance
multidisciplinary
Myosin
Peptides
Plastic surgery
Protein interaction
Protein Interaction Maps
Proteins
Proteome - metabolism
Proteomics
Proteomics - methods
Retinoic acid
Science
Science (multidisciplinary)
Therapeutic applications
Tretinoin - metabolism
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Description
Summary:Cleft palate (CP) is a congenital condition characterized by a complex etiology and limited diagnostic and therapeutic options. In this study, we delved into the molecular mechanisms associated with retinoic acid (RA)-induced CP in Kun Ming mice. Proteomic analysis of control and RA-induced CP samples at embryonic day 15.5 revealed 25 upregulated and 19 downregulated proteins. Further analysis identified these differentially expressed proteins (DEPs) as being involved in extracellular matrix organization, actin cytoskeleton, and myosin complex. Moreover, these DEPs were found to be enriched in pathways related to motor protein activity and extracellular matrix-receptor interaction. Protein-protein interaction network analysis identified 10 hub proteins, including motor proteins and ECM-related proteins, which exhibited higher expression levels in CP compared to control tissues. These findings provide insights into the molecular mechanisms underlying CP and highlight potential targets for diagnostic and therapeutic purposes.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-024-73036-0