Three-dimensional ultrastructural analysis of the interface between an implanted demineralised dentin matrix and the surrounding newly formed bone

Previous investigators have reported that transplanted demineralised dentin matrix (DDM) influences bone formation in vivo . However, the specific mechanism of how dentinal tubules contribute to bone formation has not been determined with regard to DDM transplantation therapy. In this study, we ultr...

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Bibliographic Details
Published in:Scientific reports 2018-02, Vol.8 (1), p.2858-9, Article 2858
Main Authors: Tanoue, Ryuichiro, Ohta, Keisuke, Miyazono, Yoshihiro, Iwanaga, Joe, Koba, Akihiro, Natori, Toru, Iwamoto, Osamu, Nakamura, Kei-ichiro, Kusukawa, Jingo
Format: Article
Language:English
Subjects:
14/28
14/63
631/136/818
692/698/3008
Animals
Bone growth
Bone implants
Bone matrix
Bone Regeneration
Bones
Computed tomography
Dentin - transplantation
Dentin - ultrastructure
Humanities and Social Sciences
Humans
Ion beams
Male
Microscopy, Electron, Scanning
multidisciplinary
Osteocytes
Osteocytes - metabolism
Osteocytes - ultrastructure
Osteogenesis
Rats
Scanning electron microscopy
Science
Science (multidisciplinary)
Skull - growth & development
Skull - injuries
Skull - ultrastructure
Teeth
Transplantation
Transplants & implants
Tubules
X-Ray Microtomography
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Summary:Previous investigators have reported that transplanted demineralised dentin matrix (DDM) influences bone formation in vivo . However, the specific mechanism of how dentinal tubules contribute to bone formation has not been determined with regard to DDM transplantation therapy. In this study, we ultrastructurally investigated how DDM contacted the surrounding newly formed bone using a scanning electron microscopy (SEM) three-dimensional reconstruction method that is based on focused ion beam slicing and SEM (FIB/SEM). A pulverised and processed DDM derived from human teeth was implanted into rat calvarial bone defects, and a series of X-ray computed tomographic images were obtained over 12 weeks. Implants with surrounding new bone were removed and histologically examined using FIB/SEM. After obtaining objective block-face images, the target boundary face was reconstructed three-dimensionally. The osteocytes of the new bone tissue surrounding the DDM formed a network connected by their cellular processes and formed bone tissue. It is also interesting that the cellular processes of the osteocytes extended into the dentinal tubules, and that bone tissue with canaliculi had formed and filled the DDM surface.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-018-21291-3