First report of Longidorus leptocephalus Hooper, 1961 (Nematoda: Longidoridae) from Greece

Longidorid nematodes comprise more than 500 species, and and are the most diversified, prevalent, and cosmopolitan genera within plant-parasitic nematodes. The genus comprise a group of species, some of which are vectors of plant viruses. New sampling for needle nematodes was carried out in a grapev...

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Bibliographic Details
Published in:Journal of nematology 2022-07, Vol.54 (1), p.20220027-20220027
Main Authors: Clavero-Camacho, Ilenia, Cantalapiedra-Navarrete, Carolina, Kormpi, Maria, Palomares-Rius, Juan E., Tzortzakakis, Emmanuel A., Castillo, Pablo, Archidona-Yuste, Antonio
Format: Article
Language:English
Subjects:
cytochrome oxidase c subunit 1
D2-D3 of 28S rDNA
description
ITS1 rDNA
needle nematodes
Research Paper
taxonomy
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Summary:Longidorid nematodes comprise more than 500 species, and and are the most diversified, prevalent, and cosmopolitan genera within plant-parasitic nematodes. The genus comprise a group of species, some of which are vectors of plant viruses. New sampling for needle nematodes was carried out in a grapevine area in Thessaloniki, northern Greece, and one nematode species of ( ) was recovered. Nematodes were extracted from soil samples by modified sieving and a decanting method. Extracted specimens were processed using glycerol, mounted on permanent slides, and subsequently identified morphologically. Nematode DNA was extracted from individual, live specimens, and PCR (Polymerase Chain Reaction) assays were performed for D2-D3 expansion segments of 28S rRNA, ITS1 rRNA, and partial mitochondrial regions. Morphology and morphometric data obtained from this population were consistent with the original description and reported populations of . To our knowledge, this is the first report of in Greece and the second in the Mediterranean Basin after the record of the species from Slovenia, extending the geographical distribution of this species in Europe.
ISSN:2640-396X
0022-300X
2640-396X
DOI:10.2478/jofnem-2022-0027