High-performance liquid chromatographic quantification of flavonoids in Eriocaulaceae species and their antimicrobial activity

Quantification of prepared samples by analysis using high performance liquid chromatography with DAD detection was developed to analyze rutin, 6-methoxyapigenin, and 6-methoxyapigenin-7-O-beta-D-glucopyranoside isolated from methanolic extracts of different genus: Syngonanthus, Leiothix and Eriocaul...

Full description

Saved in:
Bibliographic Details
Published in:Molecules (Basel, Switzerland) Switzerland), 2009-11, Vol.14 (11), p.4644-4654
Main Authors: da Silva, Marcelo Aparecido, Cardoso, Claudia Andréa Lima, Vilegas, Wagner, dos Santos, Lourdes Campaner
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents - chemistry
Anti-Bacterial Agents - pharmacology
Antifungal Agents - chemistry
Antifungal Agents - pharmacology
Antimicrobial agents
Bacteria - drug effects
Calibration
Candida albicans - drug effects
Chromatography
Chromatography, High Pressure Liquid
E coli
eriocaulaceae
Eriocaulaceae - chemistry
Flavonoids
Flavonoids - chemistry
Flavonoids - pharmacology
Fungi - drug effects
liquid chromatography
Microbial Sensitivity Tests
Microorganisms
Molecular Structure
Plant Extracts - chemistry
Plant Extracts - pharmacology
Pseudomonas aeruginosa - drug effects
quantification
Regression analysis
Saccharomyces cerevisiae - drug effects
Salmonella
Staphylococcus aureus - drug effects
Yeast
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Quantification of prepared samples by analysis using high performance liquid chromatography with DAD detection was developed to analyze rutin, 6-methoxyapigenin, and 6-methoxyapigenin-7-O-beta-D-glucopyranoside isolated from methanolic extracts of different genus: Syngonanthus, Leiothix and Eriocaulon (Eriocaulaceae). The linearity, accuracy, and the inter-day precision of the procedure were evaluated. The calibration curves were linear. The recoveries of the flavonoids in the samples analyzed were 96.3% to 98.5%. The percentage coefficient of variation for the quantitative analysis of the flavonoids in the analyses of the samples was under 5%. The antimicrobial activity of the five methanol extracts of these Eriocaulaceae species was assayed against the microorganisms Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Salmonella setubal, Saccharomyces cerevisiae and Candida albicans. Measured MIC values ranged from 1.25 to 10.00 mg/mL. The flavonoid contents suggest that Eriocaulaceae species may be a promising source of compounds to produce natural phytomedicines.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules14114644