Ganoderma lucidum polysaccharide improves rat DSS-induced colitis by altering cecal microbiota and gene expression of colonic epithelial cells

• Published in: Food & nutrition research 2019, Vol.63, p.1-12
• Main Authors: Xie, Jinli, Liu, Yanghanxiu, Chen, Bohui, Zhang, Guangwen, Ou, Shiyi, Luo, Jianming, Peng, Xichun
• Format: Article
• Language: English
• Subjects: colitis; colonic epithelial expression; Ganoderma lucidum polysaccharide; gut microbiota; Original; short-chain fatty acids
• ISSN: 1654-661X; 1654-661X
• DOI: 10.29219/fnr.v63.1559

The effects of β-glucan on colitis mice are contradictory in previous reports. As a result, it is still unclear whether there is an anti-colitis effect in polysaccharide (GLP), which is mainly composed of β-glucan. Moreover, the association between GLP function and gut microbiota remains to be elucidated. This study aimed to investigate whether GLP consumption improved rat dextran sodium sulfate (DSS)-induced colitis by regulating gut microbiota and altering colonic epithelial expression. The disease activity index (DAI) scores and the cecal short chain fatty acid (SCFA) levels of DSS-induced colitis rats fed with a GLP diet (Group GLP, = 6) and a control diet (Group Con, = 6) were investigated and analyzed. Moreover, the profiles of gut microbiota and colonic epithelial expression were analyzed using metagenomics and transcriptomics. GLP consumption significantly lowered animal DAI scores by producing more SCFAs by increasing SCFA-producing bacteria such as and reducing pathogens such as in both the small intestine and cecum of rat. Moreover, GLP consumption regulated 11 genes, including six upregulated ( , , , , , and ) and five downregulated ( , , , , and ) genes enriched in six inflammation-related Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, resulting in enhancement of immunity and reduction of inflammatory response and colonic cancer risk. GLP consumption alleviated DSS-induced colitis and may have potential for ulcerative colitis relief.