Detection of Increased Plasma Interleukin-6 Levels and Prevalence of Prevotella copri and Bacteroides vulgatus in the Feces of Type 2 Diabetes Patients

Intestinal dysbiosis and metabolic endotoxemia have been associated with metabolic disorders, such as obesity, insulin resistance, and type 2 diabetes (T2D). The main goal of the present study was to evaluate the intestinal dysbiosis in Brazilian T2D patients and correlate these data with inflammato...

Full description

Saved in:
Bibliographic Details
Published in:Frontiers in immunology 2017-09, Vol.8, p.1107-1107
Main Authors: Leite, Aline Zazeri, Rodrigues, Nathália de Campos, Gonzaga, Marina Ignácio, Paiolo, João Carlos Cicogna, de Souza, Carolina Arantes, Stefanutto, Nadine Aparecida Vicentini, Omori, Wellington Pine, Pinheiro, Daniel Guariz, Brisotti, João Luiz, Matheucci Junior, Euclides, Mariano, Vânia Sammartino, de Oliveira, Gislane Lelis Vilela
Format: Article
Language:English
Subjects:
dietary habits
Immunology
inflammatory cytokines
interleukin-6
intestinal microbiota
metabolic endotoxemia
type 2 diabetes
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Intestinal dysbiosis and metabolic endotoxemia have been associated with metabolic disorders, such as obesity, insulin resistance, and type 2 diabetes (T2D). The main goal of the present study was to evaluate the intestinal dysbiosis in Brazilian T2D patients and correlate these data with inflammatory cytokines and lipopolysaccharides (LPS) plasma concentrations. This study was approved by the Ethics Committees from Barretos Cancer Hospital and all individuals signed the informed consent form. Stool samples were required for DNA extraction, and the V3/V4 regions of bacterial 16S were sequenced using an Illumina platform. Peripheral blood was used to quantify inflammatory cytokines and plasma LPS concentrations, by CBA flex and ELISA, respectively. Statistical analyses were performed using Mann-Whitney and Spearman's tests. Analysis of variance, diversity indexes, and analysis of alpha- and beta-diversity were conducted using an annotated Operational Taxonomic Unit table. This study included 20 patients and 22 controls. We observed significant differences (  
ISSN:1664-3224
1664-3224
DOI:10.3389/fimmu.2017.01107