Prospective Application of Nanoencapsulated Bacillus amyloliquefaciens on Broiler Chickens' Performance and Gut Health with Efficacy against Campylobacter jejuni Colonization

• Published in: Animals (Basel) 2023-02, Vol.13 (5), p.775
• Main Authors: Ismail, Hesham, Ibrahim, Doaa, El Sayed, Shorouk, Wahdan, Ali, El-Tarabili, Reham M, Rizk El-Ghareeb, Waleed, Abdullah Alhawas, Bassam, Alahmad, Badr Abdul-Hakim Y, Abdel-Raheem, Sherief M, El-Hamid, Marwa I Abd
• Format: Article
• Language: English
• Subjects: Antibiotics; Antimicrobial agents; Bacillus amyloliquefaciens; Bacteria; Bacterial infections; barrier function; Bioavailability; Birds; Body weight; Body weight gain; C. jejuni; Campylobacter; Campylobacter jejuni; Cecum; Chickens; Cholecystokinin; Colonization; Conversion ratio; Diet; Digestive enzymes; Digestive system; Drug dosages; Enzymes; Fatty acid-binding protein; Feed additives; Feed conversion; Feeds; Food additives; Food chains; Gene expression; Genes; Homeostasis; Microbiota; Nanomaterials; Nanoparticles; Nanotechnology; Nutrition; Pathogens; Polymerase chain reaction; Poultry; probiotic-loaded nanoparticle; Probiotics; Promoters; Shedding; Tumor necrosis factor-TNF; Veterinary medicine
• ISSN: 2076-2615; 2076-2615
• DOI: 10.3390/ani13050775

Probiotics as novel antibiotics' substitutes are verified to provide barriers for hindering the colonization of enteric bacterial pathogens with nutritional benefits. For enhancement of the probiotics' effectiveness, their integration within nanomaterials is a paramount tool to support the progress of new compounds with functional features. Therefore, we addressed the impact of effective delivery of probiotics ( ) loaded nanoparticles (BNPs) on performance and ( ) shedding and colonization in poultry. Two hundred Ross broiler chickens were divided into four groups fed various BNP levels: BNPs I, BNPs II, BNPs III, and BNPs-free diets for 35 days. Nanoparticles delivery of probiotics within broiler diets improved growth performance as reflected by higher body weight gain and superior feed conversion ratio, especially in BNPs II- and BNPs III-fed groups. In parallel, the mRNA expression levels of digestive enzymes encoding genes ( , , , and ) achieved their peaks in BNPs III-fed group (1.69, 1.49, 1.33, and 1.29-fold change, respectively) versus the control one. Notably, with increasing the levels of BNPs, the abundance of beneficial microbiota, such as and species, was favored over harmful ones, including species and . Birds fed higher levels of BNPs displayed significant improvement in the expression of barrier functions-linked genes including , , and alongside substantial reduction in cecal colonization and fecal shedding of . From the aforementioned positive effects of BNPs, we concluded their potential roles as growth promoters and effective preventive aids for infection in poultry.