Impact of Storage Conditions on the Stability of Predominant Phenolic Constituents and Antioxidant Activity of Dried Piper betle Extracts

• Published in: Molecules (Basel, Switzerland) Switzerland), 2018-02, Vol.23 (2), p.484
• Main Authors: Ali, Ameena, Chong, Chien Hwa, Mah, Siau Hui, Abdullah, Luqman Chuah, Choong, Thomas Shean Yaw, Chua, Bee Lin
• Format: Article
• Language: English
• Subjects: antioxidant capacity; Antioxidants; Antioxidants - chemistry; Antioxidants - pharmacology; Constituents; Correlation coefficients; Degradation; Drug Stability; Eugenol; Free Radical Scavengers - chemistry; Free Radical Scavengers - pharmacology; Gas Chromatography-Mass Spectrometry; Isoeugenol; Kinetics; Light; Low temperature; Phenol - chemistry; Phenolic compounds; Phenols; Phytochemicals - chemistry; Piper betle; Piper betle - chemistry; Plant Extracts - chemistry; Plant Extracts - pharmacology; Shelf life; stability; Stability analysis; Storage; Storage conditions; Storage temperature; Temperature; Temperature effects; total phenolic content
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules23020484

The phenolic constituents in are well known for their antioxidant potential; however, current literature has very little information on their stability under the influence of storage factors. Present study evaluated the stability of total phenolic content (TPC) and antioxidant activity together with individual phenolic constituents (hydroxychavicol, eugenol, isoeugenol and allylpyrocatechol 3,4-diacetate) present in dried 's extract under different storage temperature of 5 and 25 °C with and without light for a period of six months. Both light and temperature significantly influenced TPC and its corresponding antioxidant activity over time. More than 95% TPC and antioxidant activity was retained at 5 °C in dark condition after 180 days of storage. Hydroxychavicol demonstrated the best stability with no degradation while eugenol and isoeugenol displayed moderate stability in low temperature (5 °C) and dark conditions. 4-allyl-1,2-diacetoxybenzene was the only compound that underwent complete degradation. A new compound, 2,4-di- -butylphenol, was detected after five weeks of storage only in the extracts exposed to light. Both zero-order and first-order kinetic models were adopted to describe the degradation kinetics of the extract's antioxidant activity. Zero-order displayed better fit with higher correlation coefficients ( ² = 0.9046) and the half-life was determined as 62 days for the optimised storage conditions (5 °C in dark conditions).