Extraction, purification and characterization of an arabinogalactan from frost (riverbank) grape (Vitis riparia Michx.) stems

This study investigated the extraction and properties of an arabinogalactan polysaccharide from frost grape (FGP) as a potential alternative to gum arabic (GA). Collection date, solvent:feed ratio (S:F), chip size, C-18 filtration, ultrafiltration, freeze drying versus spray drying, methanol pre-ext...

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Bibliographic Details
Published in:Bioresources 2023-08, Vol.18 (3), p.4610-4635
Main Authors: Eller, Fred J., Vaughn, Steven F., Price, Neil P. J., Kenar, James A., Jackson, Michael A., Berhow, Mark A., Brownstein, Korey J., Selling, Gordon W.
Format: Article
Language:English
Subjects:
Arabinogalactan
Atmospheric pressure
Chipping
Chips
Contact pressure
Diet
Dietary fiber
Emulsification
Exudates
Exudation
Filtration
Freeze drying
Frost
frost grape
Fructose
Glucose
Grapes
Methanol
Polysaccharides
pressure swing extraction
resveratrol
River banks
Spray drying
Stability analysis
Sucrose
Textiles
titanium
Ultrafiltration
Viscosity
Water absorption
Water purification
Wood preservatives
ε-viniferin
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Summary:This study investigated the extraction and properties of an arabinogalactan polysaccharide from frost grape (FGP) as a potential alternative to gum arabic (GA). Collection date, solvent:feed ratio (S:F), chip size, C-18 filtration, ultrafiltration, freeze drying versus spray drying, methanol pre-extraction, and water absorption were examined. Sugar composition, elemental analysis, dietary fiber content, emulsification activity index (EAI), emulsification stability index (ESI), and viscosity were used to evaluate the extracts. Exudates collected in March from live stems were viscous with high percentage solids and FGP, while May collections were watery with low percentage solids and FGP. Frost grape stems were collected, chipped, and classified by size. The extraction system utilized pressure or vacuum to increase contact between the chips and extraction water. A S:F ratio of ca. 24% gave an excellent yield of FGP. Chips between 1.8 and 3.8 mm gave the highest mass yields. Pre-extracting the chips with methanol and C-18 filtration of the water extract both yielded a lighter product. The EAI for the FGP was higher than that for GA; however, its ESI was lower. Ultrafiltration of the crude extract separated glucose, fructose, and sucrose from the FGP. FGP with glucose, fructose, and sucrose adsorbed water and became darker.
ISSN:1930-2126
1930-2126
DOI:10.15376/biores.18.3.4610-4635