Study on the Genetic Structure Based on Geographic Populations of the Endangered Tree Species: Liriodendron chinense

• Published in: Forests 2021-07, Vol.12 (7), p.917
• Main Authors: Zhou, Peng-Yan, Hui, Li-Xing, Huang, Shu-Jing, Ni, Zhou-Xian, Yu, Fa-Xin, Xu, Li-An
• Format: Article
• Language: English
• Subjects: Climate change; conservation biology; Differentiation; Endangered & extinct species; Endangered plants; Endangered populations; Endangered species; Gene flow; Genetic analysis; Genetic diversity; Genetic resources; Genetic structure; Geography; Heterozygosity; Inbreeding; Islands; Laboratories; Liriodendron; Liriodendron chinense; Liriodendron tulipifera; Low level; Monte Carlo simulation; Natural populations; Phylogeny; phylogeography; Plant species; Polymorphism; Population genetics; Population studies; Populations; Protected plants; Seeds; Software; species distribution pattern; Species extinction
• ISSN: 1999-4907; 1999-4907
• DOI: 10.3390/f12070917

Liriodendron chinense (Hemsley) Sargent is a Class II protected plant in China as natural populations are on the verge of extinction. There is still a lack of systematic research on the genetic resources of its geographic populations. In this study, we used 20 pairs of SSR markers with high polymorphism to analyze a total of 808 L. chinense samples from 22 regions, and 63 Liriodendron tulipifera Linn samples from 2 regions were used as a comparison group. The results revealed a total of 78 alleles in L. chinense, and the average expected heterozygosity (He) was 0.558, showing a low level of genetic diversity. The degree of differentiation of L. chinense was high, with the differentiation coefficient (Fst) as high as 0.302, which is related to the low gene flow (Nm = 0.578). Based on the genetic structure, principal coordinate analysis (PCoA) and phylogenetic analysis of 24 Liriodendron spp. populations, L. chinense and L. tulipifera had obvious differentiation, while the differentiation between L. chinense geographic populations was very large and irregular. Inbreeding appears within the geographic populations, and the level of genetic diversity is very low. In order to protect the genetic diversity of L. chinense, in addition to protecting the existing population as much as possible, artificial cultivation should introduce materials from multiple populations.