Purification of Coffee Polyphenols Extracted from Coffee Pulps ( Coffee arabica L.) Using Aqueous Two-Phase System

Coffee pulp is an abundant residue from the coffee industry, but it still contains large amounts of valuable compounds such as polyphenols. The extraction of polyphenols from coffee pulp by the conventional method is accompanied by contaminated compounds. This study, therefore, applied an aqueous tw...

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Bibliographic Details
Published in:Molecules (Basel, Switzerland) Switzerland), 2023-08, Vol.28 (15), p.5922
Main Authors: Le, Phuong Hong, Ho, Linh Thuy Thi, Le, Dao Hong Thi, Nguyen, Viet
Format: Article
Language:English
Subjects:
Alcohol
antioxidant activity
Antioxidants
aqueous two-phase system
Coffee
Coffee industry
coffee pulp extract
Communication
Efficiency
Energy consumption
Polymers
Polyphenols
purification
Salt
Solvents
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Summary:Coffee pulp is an abundant residue from the coffee industry, but it still contains large amounts of valuable compounds such as polyphenols. The extraction of polyphenols from coffee pulp by the conventional method is accompanied by contaminated compounds. This study, therefore, applied an aqueous two-phase system consisting of different ratios of ethanol/ammonium sulfate to eliminate impurities from coffee-pulp crude extract. The purification efficiency was evaluated via total polyphenol content, antioxidant activity and two major polyphenols in coffee pulps including chlorogenic acid and caffeic acid. Results showed that phenolic compounds mostly predominated in the alcohol-rich phase in which the antioxidant activity was greatly increased after the purification process. Compared to un-purified crude-coffee extract, the antioxidant activity of the purified samples increased approximately 34%, which was assumed to occur due to the slight increase of chlorogenic acid and caffeic acid. Fourier-transform infrared spectroscopy supported the effectiveness of the purification process by eliminating some impurities.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules28155922