Identification of body fluids—menstrual blood, saliva, and nasal secretions—over different periods of time, using mRNA

Background Forensic examination of biological samples started at the beginning of the twentieth century by applying the ABO blood group system in evidence related to crimes or human identification. In the present study, real-time PCR multiplex was used to identify dried and stored swabs (saliva, nas...

Full description

Saved in:
Bibliographic Details
Published in:Egyptian journal of forensic sciences 2021-12, Vol.11 (1), p.1-6, Article 42
Main Authors: Hussein, Riham F., El Mahdy, Sherif Mohamed, Saged, Nashwa Mohammed, Rashed, L., Ghaleb, Sherien S.
Format: Article
Language:English
Subjects:
Forensic Medicine
Medicine
Medicine & Public Health
Menstrual blood
Menstruation
Nasal secretions
Original Article
Pathology
Real-time PCR multiplex
Saliva
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by
cites cdi_FETCH-LOGICAL-c380t-11e7533ccb72281e025281f5f36590c2da475667b687d062a1e8229fc079e8b3
container_end_page 6
container_issue 1
container_start_page 1
container_title Egyptian journal of forensic sciences
container_volume 11
creator Hussein, Riham F.
El Mahdy, Sherif Mohamed
Saged, Nashwa Mohammed
Rashed, L.
Ghaleb, Sherien S.
description Background Forensic examination of biological samples started at the beginning of the twentieth century by applying the ABO blood group system in evidence related to crimes or human identification. In the present study, real-time PCR multiplex was used to identify dried and stored swabs (saliva, nasal secretions, and menstrual blood) through the target genes of saliva (histatin 3 and statherin), nasal secretions (statherin and BPIFA1), and menstrual blood (metalloproteinases 10 and 7). Results The expressions of histatin 3 and statherin in the dried saliva decreased over days of storage with a significant p value of
doi_str_mv 10.1186/s41935-021-00256-0
format article
fullrecord <record><control><sourceid>proquest_doaj_</sourceid><recordid>TN_cdi_doaj_primary_oai_doaj_org_article_4219a995e8034c19bc640b503ed9d19d</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_4219a995e8034c19bc640b503ed9d19d</doaj_id><sourcerecordid>2605425784</sourcerecordid><originalsourceid>FETCH-LOGICAL-c380t-11e7533ccb72281e025281f5f36590c2da475667b687d062a1e8229fc079e8b3</originalsourceid><addsrcrecordid>eNp9kcFq3DAQhk1oISHNC-QkyHXdjiRLlo4htM1CaKHkLmRptGjxWlvJDqSnPkSfsE9SbVzanjqXGYn__0bib5prCm8pVfJd6ajmogVGWwAmZAtnzQUDDa3QXL_6Zz5vrkrZQy0JVHX0ovm29TjNMURn55gmkgIZkn8mYVyiLz-__zjgVOa82JEMY0p-Q4od45PdEDt5Mtl6IgVdxpP7pE9PmImPIWCuYHLEHJMvJ-4cD7ghS4nTjhy-fLp907wOdix49btfNo8f3j_e3bcPnz9u724fWscVzC2l2AvOnRt6xhTF-sPagghcCg2Oedv1Qsp-kKr3IJmlqBjTwUGvUQ38stmuWJ_s3hxzPNj8bJKN5uUi5Z2xeY5uRNMxqq3WAhXwzlE9ONnBIICj155qX1k3K-uY09cFy2z2aclTfb1hEkTHRK-6qmKryuVUSsbwZysFc0rMrImZmph5ScxANfHVVKp42mH-i_6P6xcA6poA</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2605425784</pqid></control><display><type>article</type><title>Identification of body fluids—menstrual blood, saliva, and nasal secretions—over different periods of time, using mRNA</title><source>Criminology Collection</source><source>Publicly Available Content Database</source><source>Springer Nature - SpringerLink Journals - Fully Open Access</source><source>Social Science Premium Collection (Proquest) (PQ_SDU_P3)</source><creator>Hussein, Riham F. ; El Mahdy, Sherif Mohamed ; Saged, Nashwa Mohammed ; Rashed, L. ; Ghaleb, Sherien S.</creator><creatorcontrib>Hussein, Riham F. ; El Mahdy, Sherif Mohamed ; Saged, Nashwa Mohammed ; Rashed, L. ; Ghaleb, Sherien S.</creatorcontrib><description>Background Forensic examination of biological samples started at the beginning of the twentieth century by applying the ABO blood group system in evidence related to crimes or human identification. In the present study, real-time PCR multiplex was used to identify dried and stored swabs (saliva, nasal secretions, and menstrual blood) through the target genes of saliva (histatin 3 and statherin), nasal secretions (statherin and BPIFA1), and menstrual blood (metalloproteinases 10 and 7). Results The expressions of histatin 3 and statherin in the dried saliva decreased over days of storage with a significant p value of &lt;0.001. BPIFA1 was highly expressed in nasal secretions, and the expression level significantly decreased throughout the study with a significant p value of &lt;0.001. The MMP7 and MMP10 genes were highly expressed in the menstrual blood, and the expression level decreased over days of storage with a significant p value of p &lt;0.001. Conclusions Dried swabs of the saliva, Nasal secretions, Menstrual blood can be identified over the storage duration of the study using mRNA profiling of specific markers.</description><identifier>ISSN: 2090-5939</identifier><identifier>ISSN: 2090-536X</identifier><identifier>EISSN: 2090-5939</identifier><identifier>DOI: 10.1186/s41935-021-00256-0</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Forensic Medicine ; Medicine ; Medicine &amp; Public Health ; Menstrual blood ; Menstruation ; Nasal secretions ; Original Article ; Pathology ; Real-time PCR multiplex ; Saliva</subject><ispartof>Egyptian journal of forensic sciences, 2021-12, Vol.11 (1), p.1-6, Article 42</ispartof><rights>The Author(s) 2021</rights><rights>The Author(s) 2021. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c380t-11e7533ccb72281e025281f5f36590c2da475667b687d062a1e8229fc079e8b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.proquest.com/docview/2605425784?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,21376,21394,25753,27924,27925,33611,33769,37012,43733,43814,44590</link.rule.ids></links><search><creatorcontrib>Hussein, Riham F.</creatorcontrib><creatorcontrib>El Mahdy, Sherif Mohamed</creatorcontrib><creatorcontrib>Saged, Nashwa Mohammed</creatorcontrib><creatorcontrib>Rashed, L.</creatorcontrib><creatorcontrib>Ghaleb, Sherien S.</creatorcontrib><title>Identification of body fluids—menstrual blood, saliva, and nasal secretions—over different periods of time, using mRNA</title><title>Egyptian journal of forensic sciences</title><addtitle>Egypt J Forensic Sci</addtitle><description>Background Forensic examination of biological samples started at the beginning of the twentieth century by applying the ABO blood group system in evidence related to crimes or human identification. In the present study, real-time PCR multiplex was used to identify dried and stored swabs (saliva, nasal secretions, and menstrual blood) through the target genes of saliva (histatin 3 and statherin), nasal secretions (statherin and BPIFA1), and menstrual blood (metalloproteinases 10 and 7). Results The expressions of histatin 3 and statherin in the dried saliva decreased over days of storage with a significant p value of &lt;0.001. BPIFA1 was highly expressed in nasal secretions, and the expression level significantly decreased throughout the study with a significant p value of &lt;0.001. The MMP7 and MMP10 genes were highly expressed in the menstrual blood, and the expression level decreased over days of storage with a significant p value of p &lt;0.001. Conclusions Dried swabs of the saliva, Nasal secretions, Menstrual blood can be identified over the storage duration of the study using mRNA profiling of specific markers.</description><subject>Forensic Medicine</subject><subject>Medicine</subject><subject>Medicine &amp; Public Health</subject><subject>Menstrual blood</subject><subject>Menstruation</subject><subject>Nasal secretions</subject><subject>Original Article</subject><subject>Pathology</subject><subject>Real-time PCR multiplex</subject><subject>Saliva</subject><issn>2090-5939</issn><issn>2090-536X</issn><issn>2090-5939</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>ALSLI</sourceid><sourceid>BGRYB</sourceid><sourceid>M0O</sourceid><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNp9kcFq3DAQhk1oISHNC-QkyHXdjiRLlo4htM1CaKHkLmRptGjxWlvJDqSnPkSfsE9SbVzanjqXGYn__0bib5prCm8pVfJd6ajmogVGWwAmZAtnzQUDDa3QXL_6Zz5vrkrZQy0JVHX0ovm29TjNMURn55gmkgIZkn8mYVyiLz-__zjgVOa82JEMY0p-Q4od45PdEDt5Mtl6IgVdxpP7pE9PmImPIWCuYHLEHJMvJ-4cD7ghS4nTjhy-fLp907wOdix49btfNo8f3j_e3bcPnz9u724fWscVzC2l2AvOnRt6xhTF-sPagghcCg2Oedv1Qsp-kKr3IJmlqBjTwUGvUQ38stmuWJ_s3hxzPNj8bJKN5uUi5Z2xeY5uRNMxqq3WAhXwzlE9ONnBIICj155qX1k3K-uY09cFy2z2aclTfb1hEkTHRK-6qmKryuVUSsbwZysFc0rMrImZmph5ScxANfHVVKp42mH-i_6P6xcA6poA</recordid><startdate>20211202</startdate><enddate>20211202</enddate><creator>Hussein, Riham F.</creator><creator>El Mahdy, Sherif Mohamed</creator><creator>Saged, Nashwa Mohammed</creator><creator>Rashed, L.</creator><creator>Ghaleb, Sherien S.</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><general>SpringerOpen</general><scope>C6C</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>0-V</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8AM</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ALSLI</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BGRYB</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K7.</scope><scope>K9.</scope><scope>M0O</scope><scope>M0S</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>DOA</scope></search><sort><creationdate>20211202</creationdate><title>Identification of body fluids—menstrual blood, saliva, and nasal secretions—over different periods of time, using mRNA</title><author>Hussein, Riham F. ; El Mahdy, Sherif Mohamed ; Saged, Nashwa Mohammed ; Rashed, L. ; Ghaleb, Sherien S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c380t-11e7533ccb72281e025281f5f36590c2da475667b687d062a1e8229fc079e8b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Forensic Medicine</topic><topic>Medicine</topic><topic>Medicine &amp; Public Health</topic><topic>Menstrual blood</topic><topic>Menstruation</topic><topic>Nasal secretions</topic><topic>Original Article</topic><topic>Pathology</topic><topic>Real-time PCR multiplex</topic><topic>Saliva</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hussein, Riham F.</creatorcontrib><creatorcontrib>El Mahdy, Sherif Mohamed</creatorcontrib><creatorcontrib>Saged, Nashwa Mohammed</creatorcontrib><creatorcontrib>Rashed, L.</creatorcontrib><creatorcontrib>Ghaleb, Sherien S.</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>CrossRef</collection><collection>ProQuest Social Sciences Premium Collection</collection><collection>ProQuest Central (Corporate)</collection><collection>Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Criminal Justice Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>Social Science Premium Collection (Proquest) (PQ_SDU_P3)</collection><collection>ProQuest Central Essentials</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>Criminology Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Criminal Justice (Alumni)</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>Criminal Justice Database</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Egyptian journal of forensic sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hussein, Riham F.</au><au>El Mahdy, Sherif Mohamed</au><au>Saged, Nashwa Mohammed</au><au>Rashed, L.</au><au>Ghaleb, Sherien S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of body fluids—menstrual blood, saliva, and nasal secretions—over different periods of time, using mRNA</atitle><jtitle>Egyptian journal of forensic sciences</jtitle><stitle>Egypt J Forensic Sci</stitle><date>2021-12-02</date><risdate>2021</risdate><volume>11</volume><issue>1</issue><spage>1</spage><epage>6</epage><pages>1-6</pages><artnum>42</artnum><issn>2090-5939</issn><issn>2090-536X</issn><eissn>2090-5939</eissn><abstract>Background Forensic examination of biological samples started at the beginning of the twentieth century by applying the ABO blood group system in evidence related to crimes or human identification. In the present study, real-time PCR multiplex was used to identify dried and stored swabs (saliva, nasal secretions, and menstrual blood) through the target genes of saliva (histatin 3 and statherin), nasal secretions (statherin and BPIFA1), and menstrual blood (metalloproteinases 10 and 7). Results The expressions of histatin 3 and statherin in the dried saliva decreased over days of storage with a significant p value of &lt;0.001. BPIFA1 was highly expressed in nasal secretions, and the expression level significantly decreased throughout the study with a significant p value of &lt;0.001. The MMP7 and MMP10 genes were highly expressed in the menstrual blood, and the expression level decreased over days of storage with a significant p value of p &lt;0.001. Conclusions Dried swabs of the saliva, Nasal secretions, Menstrual blood can be identified over the storage duration of the study using mRNA profiling of specific markers.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><doi>10.1186/s41935-021-00256-0</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 2090-5939
ispartof Egyptian journal of forensic sciences, 2021-12, Vol.11 (1), p.1-6, Article 42
issn 2090-5939
2090-536X
2090-5939
language eng
recordid cdi_doaj_primary_oai_doaj_org_article_4219a995e8034c19bc640b503ed9d19d
source Criminology Collection; Publicly Available Content Database; Springer Nature - SpringerLink Journals - Fully Open Access; Social Science Premium Collection (Proquest) (PQ_SDU_P3)
subjects Forensic Medicine
Medicine
Medicine & Public Health
Menstrual blood
Menstruation
Nasal secretions
Original Article
Pathology
Real-time PCR multiplex
Saliva
title Identification of body fluids—menstrual blood, saliva, and nasal secretions—over different periods of time, using mRNA
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-29T16%3A55%3A19IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_doaj_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Identification%20of%20body%20fluids%E2%80%94menstrual%20blood,%20saliva,%20and%20nasal%20secretions%E2%80%94over%20different%20periods%20of%20time,%20using%20mRNA&rft.jtitle=Egyptian%20journal%20of%20forensic%20sciences&rft.au=Hussein,%20Riham%20F.&rft.date=2021-12-02&rft.volume=11&rft.issue=1&rft.spage=1&rft.epage=6&rft.pages=1-6&rft.artnum=42&rft.issn=2090-5939&rft.eissn=2090-5939&rft_id=info:doi/10.1186/s41935-021-00256-0&rft_dat=%3Cproquest_doaj_%3E2605425784%3C/proquest_doaj_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c380t-11e7533ccb72281e025281f5f36590c2da475667b687d062a1e8229fc079e8b3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2605425784&rft_id=info:pmid/&rfr_iscdi=true