FTA-LAMP based biosensor for a rapid in-field detection of Globodera pallida -the pale potato cyst nematode

• Published in: Frontiers in bioengineering and biotechnology 2024, Vol.12, p.1337879
• Main Authors: Camacho, Maria João, Albuquerque, Débora C, Inácio, Maria L, Martins, Verónica C, Mota, Manuel, Freitas, Paulo P, de Andrade, Eugénia
• Format: Article
• Language: English
• Subjects: Bioengineering and Biotechnology; FTA-card; Lab-on-a-chip; LAMP; magnetoresistive biochips; PCN
• ISSN: 2296-4185; 2296-4185
• DOI: 10.3389/fbioe.2024.1337879

The combination of a sensitive and specific magnetoresistive sensing device with an easy DNA extraction method and a rapid isothermal amplification is presented here targeting the on-site detection of , a potato endoparasitic nematode. FTA-cards were used for DNA extraction, LAMP was the method developed for DNA amplification and a nanoparticle functionalized magnetic-biosensor was used for the detection. The combinatorial effect of these three emerging technologies has the capacity to detect with a detection limit of one juvenile, even when mixed with other related species. This combined system is far more interesting than what a single technology can provide. Magnetic biosensors can be combined with any DNA extraction protocol and LAMP forming a new solution to target . The probe designed in this study consistently distinguished (∆V /V above 1%) from other cyst nematodes (∆V /V below 1%). It was confirmed that DNA either extracted with FTA-cards or Lab extraction Kit was of enough quantity and quality to detect whenever present (alone or in mixed samples), ensuring probe specificity and sensitivity. This work provides insights for a new strategy to construct advanced devices for pathogens in-field diagnostics. LAMP runs separately but can be easily integrated into a single device.