Protoporphyrin IX Based All-Solid-State Ion-Selective Electrodes for Choline Determination In Vitro

Protoporphyrin IX-based all-solid-state choline (Ch) ion-selective electrodes (ISEs) were fabricated and characterized. Poly (3,4-ethylene dioxythiophene) doped with poly (styrene sulfonate) (PEDOT/PSS) functioning as an ion-to-electron transducer was electropolymerized on the gold wire (0.5 mm diam...

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Bibliographic Details
Published in:Applied sciences 2021-06, Vol.11 (12), p.5549
Main Authors: Wang, You, Ma, Siyuan, Zou, Hongqun, Wu, Zhenyu, Luo, Zhiyuan, Li, Guang
Format: Article
Language:English
Subjects:
all-solid-state
Alzheimer's disease
artificial cerebrospinal fluid
Cerebrospinal fluid
Chloride
Choline
choline determination
Conducting polymers
Electrodes
Electrolytes
Enzymes
Ion-selective electrodes
Life span
Polymers
Potassium
potentiometric
Protoporphyrin
Protoporphyrin IX
Response time
Selectivity
Sodium
Solid state
Styrene
Wire
Work stations
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Summary:Protoporphyrin IX-based all-solid-state choline (Ch) ion-selective electrodes (ISEs) were fabricated and characterized. Poly (3,4-ethylene dioxythiophene) doped with poly (styrene sulfonate) (PEDOT/PSS) functioning as an ion-to-electron transducer was electropolymerized on the gold wire (0.5 mm diameter). The conductive polymer was covered with a Ch selective membrane containing protoporphyrin IX as an ionophore, which exhibited a lower detection limit of 0.49 μM with the potentiometric method. The Ch sensor performed a wide linear range from 1 μM to 1 mM, a fast response time of less than 5 s, and a decent selectivity of common inorganic and organic ions in the human body. Characteristics such as pH and temperature stability, life span, reproducibility and repeatability were also investigated to be satisfied. With the background of artificial cerebrospinal fluid, the recovery rate in 10−5 M of Ch solution was measured by the standard addition method, revealing the potential for biological application.
ISSN:2076-3417
2076-3417
DOI:10.3390/app11125549