Exploring microbial diversity in hot springs of Surajkund, India through 16S rRNA analysis and thermozyme characterization from endogenous isolates

Hot springs are a valuable source of biologically significant chemicals due to their high microbial diversity. To investigate the possibilities for industrial uses of these bacteria, researchers collected water and sediment samples from variety of hot springs. Our investigation employed both culture...

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Bibliographic Details
Published in:Scientific reports 2023-08, Vol.13 (1), p.14221-14221, Article 14221
Main Authors: Soy, S., Lakra, U., Prakash, P., Suravajhala, P., Nigam, V. K., Sharma, S. R., Bayal, N.
Format: Article
Language:English
Subjects:
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Agricultural wastes
Bacteria
Cell culture
Cellulase
Genetic testing
High temperature
Hot springs
Humanities and Social Sciences
Industrial applications
Metabolism
Microbiota
Microorganisms
multidisciplinary
Research methodology
Retention time
rRNA 16S
Science
Science (multidisciplinary)
Sediment samplers
Sediments
Temperature
Water springs
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Summary:Hot springs are a valuable source of biologically significant chemicals due to their high microbial diversity. To investigate the possibilities for industrial uses of these bacteria, researchers collected water and sediment samples from variety of hot springs. Our investigation employed both culture-dependent and culture-independent techniques, including 16S-based marker gene analysis of the microbiota from the hot springs of Surajkund, Jharkhand. In addition, we cultivated thermophilic isolates and screened for their ability to produce amylase, xylanase, and cellulase. After the optimized production of amylase the enzyme was partially purified and characterized using UPLC, DLS-ZP, and TGA. The retention time for the amylase was observed to be around 0.5 min. We confirmed the stability of the amylase at higher temperatures through observation of a steady thermo gravimetric profile at 400 °C. One of the thermophilic isolates obtained from the kund, demonstrated the potential to degrade lignocellulosic agricultural waste.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-023-41515-5