Huanglian Decoction treats Henoch-Schonlein purpura nephritis by inhibiting NF-κB/NLRP3 signaling pathway and reducing renal IgA deposition

Henoch-Schonlein purpura nephritis (HSPN) is a systemic vascular inflammatory disease. Huanglian Decoction (HLD) ameliorates renal injury in nephritis; however, the mechanism of action of HLD on HSPN has not been investigated. This study aimed to investigate the protective mechanism of HLD treatment...

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Published in:Anais da Academia Brasileira de Ciências 2024, Vol.96 (1), p.e20220970-e20220970
Main Authors: Hu, Lian, Li, Linlin, Che, Hong, Zhao, Bingjie, Xiao, L I, Liu, Peijia, Yi, Wenjing, Liu, Songshan
Format: Article
Language:English
Subjects:
Animals
Drugs, Chinese Herbal
Henoch-Schonlein purpura nephritis
Huanglian Decoction
IgA deposition
IgA Vasculitis - drug therapy
Immunoglobulin A
Kidney
Mice
MULTIDISCIPLINARY SCIENCES
Nephritis - drug therapy
NF-kappa B
NF-KappaB Inhibitor alpha
NF-κB/NLRP3 signaling pathway
NLR Family, Pyrin Domain-Containing 3 Protein
Signal Transduction
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Summary:Henoch-Schonlein purpura nephritis (HSPN) is a systemic vascular inflammatory disease. Huanglian Decoction (HLD) ameliorates renal injury in nephritis; however, the mechanism of action of HLD on HSPN has not been investigated. This study aimed to investigate the protective mechanism of HLD treatment in HSPN. The effects of HLD on HSPN biochemical indices, kidney injury and NF-κB/NLRP3 signaling pathway were analyzed by biochemical analysis, ELISA, HE and PAS staining, immunohistochemistry, immunofluorescence, and Western Blot. In addition, the effects of HLD on HSPN cells were analyzed. We found that HLD treatment significantly reduced renal tissue damage, decreased the levels of IL-17, IL-18, TNF-α, and IL-1β, and increased the levels of TP and ALB in HSPN mice. It also inhibited the deposition of IgA, IgG, and C3 in kidney tissues and significantly decreased the expression of IκBα, p-IκBα, NLRP3, caspase-1, and IL-1β in kidney tissues and cells. In addition, PMA treatment inhibited the above-mentioned effects of HLD. These results suggested that HLD attenuates renal injury, IgA deposition, and inflammation in HSPN mice and its mechanism of action may be related to the inhibition of the NF-κB/NLRP3 pathway.
ISSN:0001-3765
1678-2690
1678-2690
DOI:10.1590/0001-3765202420220970