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The effect of solvents on the antioxidant activity in Caco-2 cells of Irish brown seaweed extracts prepared using accelerated solvent extraction (ASE®)
The antioxidant activities of extracts from the Irish seaweed Ascophyllum nodosum (AN), Fucus vesiculosus (FV) and Fucus serratus (FS) prepared using different solvents were assessed in Caco-2 cells. The extracts were prepared using 100% H2O (AN100, FV100, FS100), 60% ethanol (AN60e, FV60e, FS60e),...
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Published in: | Journal of functional foods 2013-04, Vol.5 (2), p.940-948 |
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creator | O’Sullivan, A.M. O’Callaghan, Y.C. O’Grady, M.N. Hayes, M. Kerry, J.P. O’Brien, N.M. |
description | The antioxidant activities of extracts from the Irish seaweed Ascophyllum nodosum (AN), Fucus vesiculosus (FV) and Fucus serratus (FS) prepared using different solvents were assessed in Caco-2 cells. The extracts were prepared using 100% H2O (AN100, FV100, FS100), 60% ethanol (AN60e, FV60e, FS60e), 80% ethanol (AN80e, FV80e, FS80e) or 60% methanol (AN60m, FV60m, FS60m) combined with an accelerated solvent extraction (ASE®) technique. The cellular antioxidant status was determined by measuring catalase (CAT) and superoxide dismutase (SOD) activity and glutathione (GSH) content. The protective effects of the extracts against H2O2 and tert-BOOH-induced DNA damage were assessed using the comet assay. AN100 and AN80e significantly protected (P |
doi_str_mv | 10.1016/j.jff.2013.02.007 |
format | article |
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The extracts were prepared using 100% H2O (AN100, FV100, FS100), 60% ethanol (AN60e, FV60e, FS60e), 80% ethanol (AN80e, FV80e, FS80e) or 60% methanol (AN60m, FV60m, FS60m) combined with an accelerated solvent extraction (ASE®) technique. The cellular antioxidant status was determined by measuring catalase (CAT) and superoxide dismutase (SOD) activity and glutathione (GSH) content. The protective effects of the extracts against H2O2 and tert-BOOH-induced DNA damage were assessed using the comet assay. AN100 and AN80e significantly protected (P < 0.05) against H2O2-induced DNA damage. AN60e, AN80e, FS100, FS80e and FV60m protected against tert-BOOH-induced DNA damage. Extracts prepared from AN, particularly those prepared using 80% aqueous ethanol, appeared to have the greatest antioxidant potential, based on their ability to protect against oxidant-induced DNA damage.</description><identifier>ISSN: 1756-4646</identifier><identifier>DOI: 10.1016/j.jff.2013.02.007</identifier><language>eng</language><publisher>Elsevier</publisher><subject>Accelerated solvent extraction (ASE®) ; Brown seaweed extract ; Caco-2 ; Cellular antioxidant ; Ethanol ; Methanol</subject><ispartof>Journal of functional foods, 2013-04, Vol.5 (2), p.940-948</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c377t-31dbad478a8c2e3d212759e6b745c80b65be31223c5886ddcd7d9a4d6cebaba43</citedby><cites>FETCH-LOGICAL-c377t-31dbad478a8c2e3d212759e6b745c80b65be31223c5886ddcd7d9a4d6cebaba43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>O’Sullivan, A.M.</creatorcontrib><creatorcontrib>O’Callaghan, Y.C.</creatorcontrib><creatorcontrib>O’Grady, M.N.</creatorcontrib><creatorcontrib>Hayes, M.</creatorcontrib><creatorcontrib>Kerry, J.P.</creatorcontrib><creatorcontrib>O’Brien, N.M.</creatorcontrib><title>The effect of solvents on the antioxidant activity in Caco-2 cells of Irish brown seaweed extracts prepared using accelerated solvent extraction (ASE®)</title><title>Journal of functional foods</title><description>The antioxidant activities of extracts from the Irish seaweed Ascophyllum nodosum (AN), Fucus vesiculosus (FV) and Fucus serratus (FS) prepared using different solvents were assessed in Caco-2 cells. The extracts were prepared using 100% H2O (AN100, FV100, FS100), 60% ethanol (AN60e, FV60e, FS60e), 80% ethanol (AN80e, FV80e, FS80e) or 60% methanol (AN60m, FV60m, FS60m) combined with an accelerated solvent extraction (ASE®) technique. The cellular antioxidant status was determined by measuring catalase (CAT) and superoxide dismutase (SOD) activity and glutathione (GSH) content. The protective effects of the extracts against H2O2 and tert-BOOH-induced DNA damage were assessed using the comet assay. AN100 and AN80e significantly protected (P < 0.05) against H2O2-induced DNA damage. AN60e, AN80e, FS100, FS80e and FV60m protected against tert-BOOH-induced DNA damage. Extracts prepared from AN, particularly those prepared using 80% aqueous ethanol, appeared to have the greatest antioxidant potential, based on their ability to protect against oxidant-induced DNA damage.</description><subject>Accelerated solvent extraction (ASE®)</subject><subject>Brown seaweed extract</subject><subject>Caco-2</subject><subject>Cellular antioxidant</subject><subject>Ethanol</subject><subject>Methanol</subject><issn>1756-4646</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>DOA</sourceid><recordid>eNo9kUlOxDAURLMAifEA7LyERYKHxE6WqMXQEhILYG392D_gKMQt20w34RQcgpPhhoZVSfV_vVpUURwxWjHK5OlYjcNQccpERXlFqdoqdplqZFnLWu4UezGOlErJBN0tPu4ekeAwoEnEDyT66QXnFImfScoXmJPzb85mJWCSe3HpnbiZLMD4khOD0xTXuWVw8ZH0wb_OJCK8IlqCbynkTCSrgCsI2XmObn7InBzDACk7m76_X5drj89uz78-Tw6K7QGmiIcb3S_uL87vFlfl9c3lcnF2XRqhVCoFsz3YWrXQGo7CcsZV06HsVd2Ylvay6VEwzoVp2lZaa6yyHdRWGuyhh1rsF8tfrvUw6lVwTxDetQenfwwfHjSE5MyEuuamYZYLrhSre9N1kjPGhWw6OnDoMLPYL8sEH2PA4Z_HqF5Po0edp9HraTTlOk8jvgG4boeo</recordid><startdate>201304</startdate><enddate>201304</enddate><creator>O’Sullivan, A.M.</creator><creator>O’Callaghan, Y.C.</creator><creator>O’Grady, M.N.</creator><creator>Hayes, M.</creator><creator>Kerry, J.P.</creator><creator>O’Brien, N.M.</creator><general>Elsevier</general><scope>AAYXX</scope><scope>CITATION</scope><scope>DOA</scope></search><sort><creationdate>201304</creationdate><title>The effect of solvents on the antioxidant activity in Caco-2 cells of Irish brown seaweed extracts prepared using accelerated solvent extraction (ASE®)</title><author>O’Sullivan, A.M. ; O’Callaghan, Y.C. ; O’Grady, M.N. ; Hayes, M. ; Kerry, J.P. ; O’Brien, N.M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c377t-31dbad478a8c2e3d212759e6b745c80b65be31223c5886ddcd7d9a4d6cebaba43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Accelerated solvent extraction (ASE®)</topic><topic>Brown seaweed extract</topic><topic>Caco-2</topic><topic>Cellular antioxidant</topic><topic>Ethanol</topic><topic>Methanol</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>O’Sullivan, A.M.</creatorcontrib><creatorcontrib>O’Callaghan, Y.C.</creatorcontrib><creatorcontrib>O’Grady, M.N.</creatorcontrib><creatorcontrib>Hayes, M.</creatorcontrib><creatorcontrib>Kerry, J.P.</creatorcontrib><creatorcontrib>O’Brien, N.M.</creatorcontrib><collection>CrossRef</collection><collection>Directory of Open Access Journals</collection><jtitle>Journal of functional foods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>O’Sullivan, A.M.</au><au>O’Callaghan, Y.C.</au><au>O’Grady, M.N.</au><au>Hayes, M.</au><au>Kerry, J.P.</au><au>O’Brien, N.M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The effect of solvents on the antioxidant activity in Caco-2 cells of Irish brown seaweed extracts prepared using accelerated solvent extraction (ASE®)</atitle><jtitle>Journal of functional foods</jtitle><date>2013-04</date><risdate>2013</risdate><volume>5</volume><issue>2</issue><spage>940</spage><epage>948</epage><pages>940-948</pages><issn>1756-4646</issn><abstract>The antioxidant activities of extracts from the Irish seaweed Ascophyllum nodosum (AN), Fucus vesiculosus (FV) and Fucus serratus (FS) prepared using different solvents were assessed in Caco-2 cells. The extracts were prepared using 100% H2O (AN100, FV100, FS100), 60% ethanol (AN60e, FV60e, FS60e), 80% ethanol (AN80e, FV80e, FS80e) or 60% methanol (AN60m, FV60m, FS60m) combined with an accelerated solvent extraction (ASE®) technique. The cellular antioxidant status was determined by measuring catalase (CAT) and superoxide dismutase (SOD) activity and glutathione (GSH) content. The protective effects of the extracts against H2O2 and tert-BOOH-induced DNA damage were assessed using the comet assay. AN100 and AN80e significantly protected (P < 0.05) against H2O2-induced DNA damage. AN60e, AN80e, FS100, FS80e and FV60m protected against tert-BOOH-induced DNA damage. Extracts prepared from AN, particularly those prepared using 80% aqueous ethanol, appeared to have the greatest antioxidant potential, based on their ability to protect against oxidant-induced DNA damage.</abstract><pub>Elsevier</pub><doi>10.1016/j.jff.2013.02.007</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Accelerated solvent extraction (ASE®) Brown seaweed extract Caco-2 Cellular antioxidant Ethanol Methanol |
title | The effect of solvents on the antioxidant activity in Caco-2 cells of Irish brown seaweed extracts prepared using accelerated solvent extraction (ASE®) |
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