A sensitive electrochemical detection of metronidazole in synthetic serum and urine samples using low-cost screen-printed electrodes modified with reduced graphene oxide and C60

Monitoring the concentration of antibiotics in body fluids is essential to optimizing the therapy and minimizing the risk of bacteria resistance, which can be made with electrochemical sensors tailored with appropriate materials. In this paper, we report on sensors made with screen-printed electrode...

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Bibliographic Details
Published in:Journal of pharmaceutical analysis 2021-10, Vol.11 (5), p.646-652
Main Authors: Materón, Elsa Maria, Wong, Ademar, Freitas, Tayane Aguiar, Faria, Ronaldo Censi, Oliveira, Osvaldo N.
Format: Article
Language:English
Subjects:
Antibiotic
Fullerene
Metronidazole
Original
Reduced graphene oxide
Screen-printed electrodes
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Summary:Monitoring the concentration of antibiotics in body fluids is essential to optimizing the therapy and minimizing the risk of bacteria resistance, which can be made with electrochemical sensors tailored with appropriate materials. In this paper, we report on sensors made with screen-printed electrodes (SPE) coated with fullerene (C60), reduced graphene oxide (rGO) and Nafion (NF) (C60-rGO-NF/SPE) to determine the antibiotic metronidazole (MTZ). Under optimized conditions, the C60-rGO-NF/SPE sensor exhibited a linear response in square wave voltammetry for MTZ concentrations from 2.5 × 10−7 to 34 × 10−6 mol/L, with a detection limit of 2.1 × 10−7 mol/L. This sensor was also capable of detecting MTZ in serum and urine, with recovery between 94% and 100%, which are similar to those of the standard chromatographic method (HPLC-UV). Because the C60-rGO-NF/SPE sensor is amenable to mass production and allows for MTZ determination with simple principles of detection, it fulfills the requirements of therapeutic drug monitoring programs. [Display omitted] •Determination of metronidazole (MTZ) in urine and synthetic serum sample.•The MTZ is a synthetic antibiotic widely used for surgical prophylaxis, in patients with burn wounds and other pathologies.•The detection of MTZ is relevant to minimize its side effects and avoid acquired resistance.•We employed fullerene C60-rGO and nafion in low-cost screen-printed electrode (SPE).•Electrochemical sensor is amenable to mass production.
ISSN:2095-1779
2214-0883
DOI:10.1016/j.jpha.2021.03.004