Membrane Perfusion of Hydrophobic Substances Around Channels Embedded in the Contact Bubble Bilayer

In fluidic biomembranes, lipids and membrane proteins diffuse restlessly, and lipid compositions change steadily. To mimic dynamic behavior of the biomembranes, a method for introducing rapid changes in the constituents in the lipid bilayer was developed. In contact bubble bilayers (CBB), as a water...

Full description

Saved in:
Bibliographic Details
Published in:Scientific reports 2017-07, Vol.7 (1), p.6857-8, Article 6857
Main Authors: Iwamoto, Masayuki, Oiki, Shigetoshi
Format: Article
Language:English
Subjects:
631/1647/1453
631/443
631/57/2270/1140
9/74
Access routes
Channel gating
Cholesterol
Cytotoxicity
Humanities and Social Sciences
Hydrophobicity
Lipids
Membrane proteins
multidisciplinary
Nystatin
Oil
Perfusion
Potassium
Potassium channels
Science
Science (multidisciplinary)
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In fluidic biomembranes, lipids and membrane proteins diffuse restlessly, and lipid compositions change steadily. To mimic dynamic behavior of the biomembranes, a method for introducing rapid changes in the constituents in the lipid bilayer was developed. In contact bubble bilayers (CBB), as a water-in-oil droplet bilayer system, the bilayer hydrophobic interior is contiguous with the bulk oil phase. Making use of this geometrical feature as an access route, hydrophobic substances were administered into the bilayer. Polytheonamide B, a cytotoxic hydrophobic peptide, was applied, and oriented incorporation and relevant single-channel current recordings were enabled. Nystatin was pre-loaded in the CBB, and sterol perfusion exhibited slow development of the macroscopic current. On the contrary, the reconstituted KcsA potassium channels immediately attenuate the channel activity when cholesterol was applied. This oil-phase route in the CBB allows rapid perfusion of hydrophobic substances around the bilayer-embedded channels during continuous recordings of channel currents.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-017-07048-4