Myxococcus xanthus Encapsulin as a Promising Platform for Intracellular Protein Delivery

Introducing a new genetically encoded material containing a photoactivatable label as a model cargo protein, based on ( ) encapsulin system stably expressed in human 293T cells. Encapsulin from is known to be a protein-based container for a ferritin-like cargo in its shell which could be replaced wi...

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Bibliographic Details
Published in:International journal of molecular sciences 2022-12, Vol.23 (24), p.15591
Main Authors: Gabashvili, Anna N, Chmelyuk, Nelly S, Sarkisova, Viktoria A, Melnikov, Pavel A, Semkina, Alevtina S, Nikitin, Aleksey A, Abakumov, Maxim A
Format: Article
Language:English
Subjects:
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Controllability
Encapsulation
encapsulins
Epidermal growth factor
Ferritin
Fluorescence
Genes
Genetic code
Humans
Hyaluronic acid
Macrophages
Microscopy
Molecular weight
Monoclonal antibodies
Myxococcus xanthus
Myxococcus xanthus - genetics
Myxococcus xanthus - metabolism
Peptides
photoactivatable label
protein delivery
Proteins
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Summary:Introducing a new genetically encoded material containing a photoactivatable label as a model cargo protein, based on ( ) encapsulin system stably expressed in human 293T cells. Encapsulin from is known to be a protein-based container for a ferritin-like cargo in its shell which could be replaced with an exogenous cargo protein, resulting in a modified encapsulin system. We replaced natural cargo with a foreign photoactivatable mCherry (PAmCherry) fluorescent protein and isolated encapsulins, containing PAmCherry, from 293T cells. Isolated encapsulin shells containing photoactivatable label can be internalized by macrophages, wherein the PAmCherry fluorescent signal remains clearly visible. We believe that a genetically encoded nanocarrier system obtained in this study, can be used as a platform for controllable delivery of protein/peptide therapeutics in vitro.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms232415591