The relationship between plasma β-hydroxybutyric acid and conjugated linoleic acid in milk as a biomarker for early diagnosis of ketosis in postpartum Polish Holstein-Friesian cows

• Published in: BMC veterinary research 2019-10, Vol.15 (1), p.367-367, Article 367
• Main Authors: Puppel, Kamila, Gołębiewski, Marcin, Solarczyk, Paweł, Grodkowski, Grzegorz, Slósarz, Jan, Kunowska-Slósarz, Małgorzata, Balcerak, Marek, Przysucha, Tomasz, Kalińska, Aleksandra, Kuczyńska, Beata
• Format: Article
• Language: English
• Subjects: 3-Hydroxybutyric Acid - blood; Animals; Biomarkers; Cattle; Cattle Diseases - blood; Cattle Diseases - metabolism; Conjugated linoleic acid; Cow; Dairying; Fatty Acids, Nonesterified - blood; Female; Ketosis; Ketosis - blood; Ketosis - diagnosis; Ketosis - veterinary; Lactation - metabolism; Linoleic Acids, Conjugated - blood; Milk; Milk - chemistry; Postpartum Period - blood; Postpartum Period - metabolism; β-Hydroxybutyric acid
• ISSN: 1746-6148; 1746-6148
• DOI: 10.1186/s12917-019-2131-2

The aim of this study was to investigate the association between plasma β-hydroxybutyric acid (BHBA) and conjugated linoleic acid in postpartum Polish Holstein-Friesian (PHF) cows. The experiment was carried out at an experimental dairy farm, where a herd of approximately 350 cows was kept. Samples were taken at six time points: between days 5-7, 8-14, 15-21, 22-28, 29-35, and 36-42, resulting in 510 samples of both milk and blood. The cows involved in the experiment were divided into two groups - ketotic and healthy - by taking into account general health symptoms, blood serum BHBA, and non-esterified fatty acids (NEFA) concentration at 5-7 days postpartum. In the first week of lactation, at 5-7 day in milk (DIM), the study showed a 53% lower level of C18:2 cis-9 trans-11 (CLA9) and an 80% lower level of C18:2 trans-10 cis-12 (CLA10) in cows with diagnosed ketosis compared to healthy cows. In the second week of lactation (8-14 DIM), a 34% lower level of CLA9 and a 54% lower level of CLA10 was found in the group of cows with BHBA levels > 1.2 mmol/L. Additionally, Pearson correlation analysis showed significant negative correlation between BHBA x CLA9 and BHBA x CLA10 in the first week of lactation: - 0.732and - 0.821, respectively. The study shows that that both CLA9 and CLA10 can be used as markers for the early diagnosis of elevated blood levels of BHBA in postpartum Polish Holstein-Friesian cows.