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A molecular signature for the G6PC3/SLC37A2/SLC37A4 interactors in glioblastoma disease progression and in the acquisition of a brain cancer stem cell phenotype
Glycogen plays an important role in glucose homeostasis and contributes to key functions related to brain cancer cell survival in glioblastoma multiforme (GBM) disease progression. Such adaptive molecular mechanism is dependent on the glycogenolytic pathway and intracellular glucose-6-phosphate (G6P...
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Published in: | Frontiers in endocrinology (Lausanne) 2023-11, Vol.14, p.1265698-1265698 |
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description | Glycogen plays an important role in glucose homeostasis and contributes to key functions related to brain cancer cell survival in glioblastoma multiforme (GBM) disease progression. Such adaptive molecular mechanism is dependent on the glycogenolytic pathway and intracellular glucose-6-phosphate (G6P) sensing by brain cancer cells residing within those highly hypoxic tumors. The involvement of components of the glucose-6-phosphatase (G6Pase) system remains however elusive.
We questioned the gene expression levels of components of the G6Pase system in GBM tissues and their functional impact in the control of the invasive and brain cancer stem cells (CSC) phenotypes.
analysis of transcript levels in GBM tumor tissues was done by GEPIA. Total RNA was extracted and gene expression of
as well as of
members analyzed by qPCR in four human brain cancer cell lines and from clinically annotated brain tumor cDNA arrays. Transient siRNA-mediated gene silencing was used to assess the impact of TGF-β-induced epithelial-to-mesenchymal transition (EMT) and cell chemotaxis. Three-dimensional (3D) neurosphere cultures were generated to recapitulate the brain CSC phenotype.
Higher expression in
,
, and
was found in GBM tumor tissues in comparison to low-grade glioma and healthy tissue. The expression of these genes was also found elevated in established human U87, U251, U118, and U138 GBM cell models compared to human HepG2 hepatoma cells.
, but not
, levels were induced in 3D CD133/SOX2-positive U87 neurospheres when compared to 2D monolayers. Silencing of
/
altered TGF-β-induced EMT biomarker SNAIL and cell chemotaxis.
Two members of the G6Pase system, G6PC3 and SLC37A4, associate with GBM disease progression and regulate the metabolic reprogramming of an invasive and CSC phenotype. Such molecular signature may support their role in cancer cell survival and chemoresistance and become future therapeutic targets. |
doi_str_mv | 10.3389/fendo.2023.1265698 |
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We questioned the gene expression levels of components of the G6Pase system in GBM tissues and their functional impact in the control of the invasive and brain cancer stem cells (CSC) phenotypes.
analysis of transcript levels in GBM tumor tissues was done by GEPIA. Total RNA was extracted and gene expression of
as well as of
members analyzed by qPCR in four human brain cancer cell lines and from clinically annotated brain tumor cDNA arrays. Transient siRNA-mediated gene silencing was used to assess the impact of TGF-β-induced epithelial-to-mesenchymal transition (EMT) and cell chemotaxis. Three-dimensional (3D) neurosphere cultures were generated to recapitulate the brain CSC phenotype.
Higher expression in
,
, and
was found in GBM tumor tissues in comparison to low-grade glioma and healthy tissue. The expression of these genes was also found elevated in established human U87, U251, U118, and U138 GBM cell models compared to human HepG2 hepatoma cells.
, but not
, levels were induced in 3D CD133/SOX2-positive U87 neurospheres when compared to 2D monolayers. Silencing of
/
altered TGF-β-induced EMT biomarker SNAIL and cell chemotaxis.
Two members of the G6Pase system, G6PC3 and SLC37A4, associate with GBM disease progression and regulate the metabolic reprogramming of an invasive and CSC phenotype. Such molecular signature may support their role in cancer cell survival and chemoresistance and become future therapeutic targets.</description><identifier>ISSN: 1664-2392</identifier><identifier>EISSN: 1664-2392</identifier><identifier>DOI: 10.3389/fendo.2023.1265698</identifier><identifier>PMID: 38034009</identifier><language>eng</language><publisher>Switzerland: Frontiers Media S.A</publisher><subject>Antiporters - genetics ; Antiporters - metabolism ; Brain - metabolism ; Brain Neoplasms - metabolism ; Endocrinology ; G6PC3 ; G6PT ; glioblastoma ; Glioblastoma - metabolism ; Glucose-6-Phosphatase - genetics ; Glucose-6-Phosphatase - metabolism ; glucose-6-phosphatase system ; Humans ; Monosaccharide Transport Proteins - genetics ; Monosaccharide Transport Proteins - metabolism ; Neoplastic Stem Cells - metabolism ; Phenotype ; SLC37A2 ; SLC37A4 ; Transforming Growth Factor beta - metabolism</subject><ispartof>Frontiers in endocrinology (Lausanne), 2023-11, Vol.14, p.1265698-1265698</ispartof><rights>Copyright © 2023 Torabidastgerdooei, Roy and Annabi.</rights><rights>Copyright © 2023 Torabidastgerdooei, Roy and Annabi 2023 Torabidastgerdooei, Roy and Annabi</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c469t-59794a16d93155ca14cee97838c5c1d98f624e7cd48231a38b3809573b2354d83</citedby><cites>FETCH-LOGICAL-c469t-59794a16d93155ca14cee97838c5c1d98f624e7cd48231a38b3809573b2354d83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10687460/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10687460/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38034009$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Torabidastgerdooei, Sima</creatorcontrib><creatorcontrib>Roy, Marie-Eve</creatorcontrib><creatorcontrib>Annabi, Borhane</creatorcontrib><title>A molecular signature for the G6PC3/SLC37A2/SLC37A4 interactors in glioblastoma disease progression and in the acquisition of a brain cancer stem cell phenotype</title><title>Frontiers in endocrinology (Lausanne)</title><addtitle>Front Endocrinol (Lausanne)</addtitle><description>Glycogen plays an important role in glucose homeostasis and contributes to key functions related to brain cancer cell survival in glioblastoma multiforme (GBM) disease progression. Such adaptive molecular mechanism is dependent on the glycogenolytic pathway and intracellular glucose-6-phosphate (G6P) sensing by brain cancer cells residing within those highly hypoxic tumors. The involvement of components of the glucose-6-phosphatase (G6Pase) system remains however elusive.
We questioned the gene expression levels of components of the G6Pase system in GBM tissues and their functional impact in the control of the invasive and brain cancer stem cells (CSC) phenotypes.
analysis of transcript levels in GBM tumor tissues was done by GEPIA. Total RNA was extracted and gene expression of
as well as of
members analyzed by qPCR in four human brain cancer cell lines and from clinically annotated brain tumor cDNA arrays. Transient siRNA-mediated gene silencing was used to assess the impact of TGF-β-induced epithelial-to-mesenchymal transition (EMT) and cell chemotaxis. Three-dimensional (3D) neurosphere cultures were generated to recapitulate the brain CSC phenotype.
Higher expression in
,
, and
was found in GBM tumor tissues in comparison to low-grade glioma and healthy tissue. The expression of these genes was also found elevated in established human U87, U251, U118, and U138 GBM cell models compared to human HepG2 hepatoma cells.
, but not
, levels were induced in 3D CD133/SOX2-positive U87 neurospheres when compared to 2D monolayers. Silencing of
/
altered TGF-β-induced EMT biomarker SNAIL and cell chemotaxis.
Two members of the G6Pase system, G6PC3 and SLC37A4, associate with GBM disease progression and regulate the metabolic reprogramming of an invasive and CSC phenotype. Such molecular signature may support their role in cancer cell survival and chemoresistance and become future therapeutic targets.</description><subject>Antiporters - genetics</subject><subject>Antiporters - metabolism</subject><subject>Brain - metabolism</subject><subject>Brain Neoplasms - metabolism</subject><subject>Endocrinology</subject><subject>G6PC3</subject><subject>G6PT</subject><subject>glioblastoma</subject><subject>Glioblastoma - metabolism</subject><subject>Glucose-6-Phosphatase - genetics</subject><subject>Glucose-6-Phosphatase - metabolism</subject><subject>glucose-6-phosphatase system</subject><subject>Humans</subject><subject>Monosaccharide Transport Proteins - genetics</subject><subject>Monosaccharide Transport Proteins - metabolism</subject><subject>Neoplastic Stem Cells - metabolism</subject><subject>Phenotype</subject><subject>SLC37A2</subject><subject>SLC37A4</subject><subject>Transforming Growth Factor beta - metabolism</subject><issn>1664-2392</issn><issn>1664-2392</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>DOA</sourceid><recordid>eNpVks1q3DAQx01paUKaF-ih6NjLbvRlWTqVZWmTwEILbc9ClsZeBdvaSHIhb5NHrZzdhESXkebjN8PoX1WfCV4zJtVVB5MLa4opWxMqaqHku-qcCMFXlCn6_tX9rLpM6Q6XwzFRSn6szpjEjGOszqvHDRrDAHYeTETJ95PJcwTUhYjyHtC1-LVlV793W9Zs6Mly5KcM0dgcYip31A8-tINJOYwGOZ_AJECHGPoIKfkwITO5JW8BGns_--Tz4g4dMqiNpoSsmSyUATKMyMIwoMMeppAfDvCp-tCZIcHlyV5Uf398_7O9We1-Xt9uN7uV5ULlVa0axQ0RTjFS19YQbgFUI5m0tSVOyU5QDo11XFJGDJNt2YGqG9ZSVnMn2UV1e-S6YO70IfrRxAcdjNdPjhB7bWL2dgDNSwusOHWuNpxjpyyRhLTWSWLLAwrr25F1mNsRnIUpRzO8gb6NTH6v-_BPEyxkwwUuhK8nQgz3M6SsR5-WxZgJwpw0lUrI8p-NKKn0mGpjSClC99KHYL1IRT9JRS9S0SeplKIvryd8KXkWBvsPTg-7eg</recordid><startdate>20231116</startdate><enddate>20231116</enddate><creator>Torabidastgerdooei, Sima</creator><creator>Roy, Marie-Eve</creator><creator>Annabi, Borhane</creator><general>Frontiers Media S.A</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20231116</creationdate><title>A molecular signature for the G6PC3/SLC37A2/SLC37A4 interactors in glioblastoma disease progression and in the acquisition of a brain cancer stem cell phenotype</title><author>Torabidastgerdooei, Sima ; Roy, Marie-Eve ; Annabi, Borhane</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c469t-59794a16d93155ca14cee97838c5c1d98f624e7cd48231a38b3809573b2354d83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Antiporters - genetics</topic><topic>Antiporters - metabolism</topic><topic>Brain - metabolism</topic><topic>Brain Neoplasms - metabolism</topic><topic>Endocrinology</topic><topic>G6PC3</topic><topic>G6PT</topic><topic>glioblastoma</topic><topic>Glioblastoma - metabolism</topic><topic>Glucose-6-Phosphatase - genetics</topic><topic>Glucose-6-Phosphatase - metabolism</topic><topic>glucose-6-phosphatase system</topic><topic>Humans</topic><topic>Monosaccharide Transport Proteins - genetics</topic><topic>Monosaccharide Transport Proteins - metabolism</topic><topic>Neoplastic Stem Cells - metabolism</topic><topic>Phenotype</topic><topic>SLC37A2</topic><topic>SLC37A4</topic><topic>Transforming Growth Factor beta - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Torabidastgerdooei, Sima</creatorcontrib><creatorcontrib>Roy, Marie-Eve</creatorcontrib><creatorcontrib>Annabi, Borhane</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>Directory of Open Access Journals</collection><jtitle>Frontiers in endocrinology (Lausanne)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Torabidastgerdooei, Sima</au><au>Roy, Marie-Eve</au><au>Annabi, Borhane</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A molecular signature for the G6PC3/SLC37A2/SLC37A4 interactors in glioblastoma disease progression and in the acquisition of a brain cancer stem cell phenotype</atitle><jtitle>Frontiers in endocrinology (Lausanne)</jtitle><addtitle>Front Endocrinol (Lausanne)</addtitle><date>2023-11-16</date><risdate>2023</risdate><volume>14</volume><spage>1265698</spage><epage>1265698</epage><pages>1265698-1265698</pages><issn>1664-2392</issn><eissn>1664-2392</eissn><abstract>Glycogen plays an important role in glucose homeostasis and contributes to key functions related to brain cancer cell survival in glioblastoma multiforme (GBM) disease progression. Such adaptive molecular mechanism is dependent on the glycogenolytic pathway and intracellular glucose-6-phosphate (G6P) sensing by brain cancer cells residing within those highly hypoxic tumors. The involvement of components of the glucose-6-phosphatase (G6Pase) system remains however elusive.
We questioned the gene expression levels of components of the G6Pase system in GBM tissues and their functional impact in the control of the invasive and brain cancer stem cells (CSC) phenotypes.
analysis of transcript levels in GBM tumor tissues was done by GEPIA. Total RNA was extracted and gene expression of
as well as of
members analyzed by qPCR in four human brain cancer cell lines and from clinically annotated brain tumor cDNA arrays. Transient siRNA-mediated gene silencing was used to assess the impact of TGF-β-induced epithelial-to-mesenchymal transition (EMT) and cell chemotaxis. Three-dimensional (3D) neurosphere cultures were generated to recapitulate the brain CSC phenotype.
Higher expression in
,
, and
was found in GBM tumor tissues in comparison to low-grade glioma and healthy tissue. The expression of these genes was also found elevated in established human U87, U251, U118, and U138 GBM cell models compared to human HepG2 hepatoma cells.
, but not
, levels were induced in 3D CD133/SOX2-positive U87 neurospheres when compared to 2D monolayers. Silencing of
/
altered TGF-β-induced EMT biomarker SNAIL and cell chemotaxis.
Two members of the G6Pase system, G6PC3 and SLC37A4, associate with GBM disease progression and regulate the metabolic reprogramming of an invasive and CSC phenotype. Such molecular signature may support their role in cancer cell survival and chemoresistance and become future therapeutic targets.</abstract><cop>Switzerland</cop><pub>Frontiers Media S.A</pub><pmid>38034009</pmid><doi>10.3389/fendo.2023.1265698</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Antiporters - genetics Antiporters - metabolism Brain - metabolism Brain Neoplasms - metabolism Endocrinology G6PC3 G6PT glioblastoma Glioblastoma - metabolism Glucose-6-Phosphatase - genetics Glucose-6-Phosphatase - metabolism glucose-6-phosphatase system Humans Monosaccharide Transport Proteins - genetics Monosaccharide Transport Proteins - metabolism Neoplastic Stem Cells - metabolism Phenotype SLC37A2 SLC37A4 Transforming Growth Factor beta - metabolism |
title | A molecular signature for the G6PC3/SLC37A2/SLC37A4 interactors in glioblastoma disease progression and in the acquisition of a brain cancer stem cell phenotype |
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