Co-continuous structural effect of size-controlled macro-porous glass membrane on extracellular vesicle collection for the analysis of miRNA

Recent studies have shown that extracellular vesicles (EVs) can be utilized as appropriate and highly specific biomarkers in liquid biopsy for the diagnosis and prognosis of serious illness. However, there are few methods that can collect and isolate miRNA in EVs simply, quickly and efficiently usin...

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Bibliographic Details
Published in:Scientific reports 2021-04, Vol.11 (1), p.8672-8672, Article 8672
Main Authors: Yukawa, Hiroshi, Yamazaki, Shuji, Aoki, Keita, Muto, Kengo, Kihara, Naoto, Sato, Kazuhide, Onoshima, Daisuke, Ochiya, Takahiro, Tanaka, Yasuhito, Baba, Yoshinobu
Format: Article
Language:English
Subjects:
631/1647/2230
631/1647/350
Adsorption
Biomarkers
Biopsy
Body fluids
Cell culture
Decomposition
Diagnosis
Extracellular vesicles
Heat resistance
Humanities and Social Sciences
Illnesses
Membranes
Metastasis
Methods
miRNA
multidisciplinary
Polyethylene glycol
Pore size
Prognosis
Proteins
Science
Science (multidisciplinary)
Silicon dioxide
Urine
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Summary:Recent studies have shown that extracellular vesicles (EVs) can be utilized as appropriate and highly specific biomarkers in liquid biopsy for the diagnosis and prognosis of serious illness. However, there are few methods that can collect and isolate miRNA in EVs simply, quickly and efficiently using general equipment such as a normal centrifuge. In this paper, we developed an advanced glass membrane column (AGC) device incorporating a size-controlled macro-porous glass (MPG) membrane with a co-continuous structure to overcome the limitations of conventional EV collection and miRNA extraction from the EVs. The size of macro-pores in the MPG membrane could be accurately controlled by changing the heating temperature and time on the basis of spinodal decomposition of B 2 O 3 , Na 2 O, and SiO 2 in phase separation. The AGC device with an MPG membrane could collect the EVs simply and quickly (
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-021-87986-2