Transcriptomic uniqueness and commonality of the ion channels and transporters in the four heart chambers

Myocardium transcriptomes of left and right atria and ventricles from four adult male C57Bl/6j mice were profiled with Agilent microarrays to identify the differences responsible for the distinct functional roles of the four heart chambers. Female mice were not investigated owing to their transcript...

Full description

Saved in:
Bibliographic Details
Published in:Scientific reports 2021-02, Vol.11 (1), p.2743-2743, Article 2743
Main Authors: Iacobas, Sanda, Amuzescu, Bogdan, Iacobas, Dumitru A.
Format: Article
Language:English
Subjects:
631/208/199
692/4019
Atrium
Calcium signalling
Cardiac muscle
DNA microarrays
Estrus cycle
Gene expression
Gluconeogenesis
Glycolysis
Humanities and Social Sciences
Ion channels
multidisciplinary
Muscle contraction
Myocardium
Oxidative phosphorylation
Phosphorylation
Science
Science (multidisciplinary)
Transcriptomes
Ventricle
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Myocardium transcriptomes of left and right atria and ventricles from four adult male C57Bl/6j mice were profiled with Agilent microarrays to identify the differences responsible for the distinct functional roles of the four heart chambers. Female mice were not investigated owing to their transcriptome dependence on the estrous cycle phase. Out of the quantified 16,886 unigenes, 15.76% on the left side and 16.5% on the right side exhibited differential expression between the atrium and the ventricle, while 5.8% of genes were differently expressed between the two atria and only 1.2% between the two ventricles. The study revealed also chamber differences in gene expression control and coordination. We analyzed ion channels and transporters, and genes within the cardiac muscle contraction, oxidative phosphorylation, glycolysis/gluconeogenesis, calcium and adrenergic signaling pathways. Interestingly, while expression of Ank2 oscillates in phase with all 27 quantified binding partners in the left ventricle, the percentage of in-phase oscillating partners of Ank2 is 15% and 37% in the left and right atria and 74% in the right ventricle. The analysis indicated high interventricular synchrony of the ion channels expressions and the substantially lower synchrony between the two atria and between the atrium and the ventricle from the same side.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-021-82383-1