Magnetic Bioreactor for Magneto-, Mechano- and Electroactive Tissue Engineering Strategies

Biomimetic bioreactor systems are increasingly being developed for tissue engineering applications, due to their ability to recreate the native cell/tissue microenvironment. Regarding bone-related diseases and considering the piezoelectric nature of bone, piezoelectric scaffolds electromechanically...

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Bibliographic Details
Published in:Sensors (Basel, Switzerland) Switzerland), 2020-06, Vol.20 (12), p.3340
Main Authors: Castro, Nelson, Fernandes, Margarida M, Ribeiro, Clarisse, Correia, Vítor, Minguez, Rikardo, Lanceros-Méndez, Senentxu
Format: Article
Language:English
Subjects:
Advantages
Biocompatibility
Biomimetics
Bioreactors
Bone and Bones
Bones
Cell culture
Cell Culture Techniques
Cell growth
Design
Design parameters
Electric contacts
Gene expression
Humans
magnetic actuator
magnetic bioreactor
Magnetic fields
Magnetic Phenomena
magnetoactive scaffolds
magnetoelectric stimulation
Scaffolds
Tissue engineering
Tissue Engineering - instrumentation
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Summary:Biomimetic bioreactor systems are increasingly being developed for tissue engineering applications, due to their ability to recreate the native cell/tissue microenvironment. Regarding bone-related diseases and considering the piezoelectric nature of bone, piezoelectric scaffolds electromechanically stimulated by a bioreactor, providing the stimuli to the cells, allows a biomimetic approach and thus, mimicking the required microenvironment for effective growth and differentiation of bone cells. In this work, a bioreactor has been designed and built allowing to magnetically stimulate magnetoelectric scaffolds and therefore provide mechanical and electrical stimuli to the cells through magnetomechanical or magnetoelectrical effects, depending on the piezoelectric nature of the scaffold. While mechanical bioreactors need direct application of the stimuli on the scaffolds, the herein proposed magnetic bioreactors allow for a remote stimulation without direct contact with the material. Thus, the stimuli application (23 mT at a frequency of 0.3 Hz) to cells seeded on the magnetoelectric, leads to an increase in cell viability of almost 30% with respect to cell culture under static conditions. This could be valuable to mimic what occurs in the human body and for application in immobilized patients. Thus, special emphasis has been placed on the control, design and modeling parameters governing the bioreactor as well as its functional mechanism.
ISSN:1424-8220
1424-8220
DOI:10.3390/s20123340