Regenerative capacity of leukocyte-rich and platelet-rich fibrin in indirect sinus elevation procedure may be dependent on model-specific modification of the centrifugation cycle

• Published in: Contemporary clinical dentistry 2019-07, Vol.10 (3), p.433-439
• Main Authors: Chandra, Rampalli, Vaishnavi, Varanasi, S. Chakravarthy, Y
• Format: Article
• Language: English
• Subjects: alveolar ridge augmentations; Analysis; Centrifugation; Centrifuges; Computer programs; Fibrin; Fibrinogen; Laboratories; Leukocytes; maxillary ridge augmentations; Optical density; Original; platelet-rich fibrin; Platelets; Sinuses; Software; Software industry; Statistical analysis; Studies
• ISSN: 0976-237X; 0976-2361
• DOI: 10.4103/ccd.ccd_715_18

Context: To compare optical density (OD) and fibrinogen content of leukocyte-rich and platelet-rich fibrin (L-PRF) generated by standard protocol (2700 rotations per minute [RPM] for 12 min) versus relative centrifugal force (RCF)-adjusted protocol across two widely used laboratory centrifuges with swing-out rotors. Aims: Centrifuges for PRF production generate forces in excess of 800 g. The study aimed to evaluate OD, fibrinogen content and effectiveness in bone-added osteotome sinus floor elevation (BAOSFE) of leukocyte-rich and platelet-rich fibrin (L-PRF) generated by the standard protocol (2700 RPM for 12 min) versus a RCF-adjusted protocol to generate precisely 400 g of force across two centrifuges with swing-out rotors. The outcomes were compared to a standard centrifuge configured to generate L-PRF as per the original Choukroun guidelines. Settings and Design: Sample size for the present study was calculated using proportional power calculation. A minimum sample size of 8 per group was needed to detect a bone height difference of 2 mm when the power of the test is 0.80 at a significance level of 0.05. Subjects and Methods: Based on the centrifuge and protocol used to generate L-PRF, 10 participants were assigned to each of the following groups are as follows: D group, fixed angle centrifuge (DUO Quattro®) at default setting. R-O group: Swing-out centrifuge (Remi 8C®) + standard protocol. R-A group: Remi 8C® centrifuge + RCF-adjusted protocol. C-O group: Swing-out centrifuge (Remi C854®) + standard protocol. and C-A group: Remi C854® + RCF-adjusted protocol. OD, fibrinogen content, and gain in bone fill and bone height after BAOSFE were the evaluated outcomes. Statistical Analysis Used: Data were analyzed using GraphPad Prism® Software version 6.0 (GraphPad Software Inc., La Jolla, USA) and SAS Software® version 9.3 versions (SAS, New Delhi, India). Data were summarized by mean ± standard deviation for continuous data and median ± inter-quartile range for the score data. The comparison between different time points was done by analysis of one-way repeated measures test, followed by post hoc test for score data. The comparison between two groups for repeated data was made by analysis of two-way repeated measures test and followed by post hoc test. Spearman's Rho correlation test was used to test the correlation between prognosis and the other variables. Results: L-PRF from the Remi C854® centrifuge with RCF-adjusted protocol showed OD (P = 0.152) and