Development of Novel Squid Gladius Biomaterials for Cornea Tissue Engineering

Cornea tissue engineering is strictly dependent on the development of biomaterials that fulfill the strict biocompatibility, biomechanical, and optical requirements of this organ. In this work, we generated novel biomaterials from the squid gladius (SG), and their application in cornea tissue engine...

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Bibliographic Details
Published in:Marine drugs 2024-11, Vol.22 (12), p.535
Main Authors: Garzón, Ingrid, Muñoz-Hurtado, Juan, Pereira-Martínez, Juan, Ionescu, Ana M, Cardona, Juan de la Cruz, Tejada-Casado, María, Pérez, María Del Mar, Campos, Fernando, Chato-Astrain, Jesús, Alaminos, Miguel
Format: Article
Language:English
Subjects:
Analysis
Animals
Biocompatibility
Biocompatible Materials - chemistry
Biocompatible Materials - pharmacology
Biodegradation
Biological products
Biomaterials
Biomechanical engineering
Biomechanics
Biomedical materials
Cartilage
Cell adhesion
Cells, Cultured
Collagen
Cornea
Cornea - drug effects
Crystallin
Decapodiformes
Deformation
Deformation analysis
Epithelial cells
Epithelium
Evaluation
Fishing
Humans
Hydrogels
Laboratory animals
Marine molluscs
Mechanical properties
natural biomaterials
Optical properties
Polyethylene glycol
Reflectance
Scanning electron microscopy
Squid
Tissue
Tissue engineering
Tissue Engineering - methods
Traction
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Summary:Cornea tissue engineering is strictly dependent on the development of biomaterials that fulfill the strict biocompatibility, biomechanical, and optical requirements of this organ. In this work, we generated novel biomaterials from the squid gladius (SG), and their application in cornea tissue engineering was evaluated. Results revealed that the native SG (N-SG) was biocompatible in laboratory animals, although a local inflammatory reaction was driven by the material. Cellularized biomaterials (C-SG) demonstrated that the SG provides an adequate substrate for cell attachment and growth, and corneal epithelial cells cultured on this biomaterial were able to express crystallin alpha, a marker for this type of cells. Biomechanical analyses showed that N-SG biomaterials have higher Young modulus and lower traction deformation than control native corneas (CTR), and C-SG showed a similar Young modulus than CTR. Analysis of the optical properties of these samples revealed that the diffuse transmittance of N-SG and C-SG were higher than CTR, with the diffuse reflectance showing the opposite behavior. These results confirm the putative usefulness of this abundant marine-derived biomaterial that can be obtained as a byproduct of the fishing industry.
ISSN:1660-3397
1660-3397
DOI:10.3390/md22120535