Ovarian cancer‐excreted exosomal miR‐199a‐5p suppresses tumor metastasis by targeting hypoxia‐inducible factor‐2α in hypoxia microenvironment

• Published in: Cancer communications (London, England) England), 2020-08, Vol.40 (8), p.380-385
• Main Authors: Lian, Xuan‐Ye, Zhang, Hui, Liu, Qun, Lu, Xue, Zhou, Ping, He, Shu‐Qian, Tang, Rui‐Xue, Cui, Jing
• Format: Article
• Language: English
• Subjects: Cancer therapies; Fallopian tubes; Genes; Genomes; Hypoxia; Letter to the Editor; Letters to the Editor; Metastasis; MicroRNAs; Nanoparticles; Ontology; Ovarian cancer; Polymerase chain reaction; Protein expression; Proteins; Transmission electron microscopy; Wound healing
• ISSN: 2523-3548; 2523-3548
• DOI: 10.1002/cac2.12034

Abbreviation EPAS1 Endothelial PAS domain-containing protein 1 GO Gene Ontology HIF-2α Hypoxia-inducible factor-2α HRF HIF-1α related factor ISH In Situ Hybridization JAM-A junctional adhesion molecule-A KEGG Kyoto Encyclopedia of Genes and Genomes NTA Nano particle tracking analysis Q-PCR Quantitative-polymerase chain reaction TDEs Tumor-derived exosomes TEM Transmission electron microscopy TME tissue microarray VE-cad VE-cadherin Dear editor, Ovarian cancer is one of the most prevalent gynecological malignant tumors. [ 7] reported that miR-199a-5p, as a tumor suppressor, could inhibit ovarian cancer cell proliferation and invasion by inhibiting the expression of NF-κB1. The Gene Ontology (GO) analysis mainly enriched the foundation of miR-199a-5p in cell-cell adhesion and transcription factor activity. TABLE 1 Correlation between the exosomal miR-199a-5p expression to the clinicopathological characteristics of ovarian cancer patients (N = 57) Relative expression of miR-199a-5p Clinicopathological characteristics Cases n High [cases, (%)] Low [cases, (%)] P Age (years) 0.574 ≤ 50 22 12 (21.1%) 10 (17.5%) > 50 35 19 (33.3%) 16 (28.1%) Tumor size(cm) < 0.001 ≤ 5 20 15 (26.3%) 5 (8.8%) > 5 37 5 (8.8%) 32 (56.1%) Infiltration < 0.001 Absent 14 11 (19.3%) 3 (5.3%) Present 43 10 (17.5%) 33 (57.9%) Lymphatic < 0.001 metastasis Absent 52 40 (70.2%) 12 (21.1%) Present 5 1 (1.7%) 4 (7.0%) T stage < 0.001 T1+T2 21 19 (33.3%) 2 (3.5%) T3+T4 36 10 (17.6%) 26 (45.6%) Wound-healing assays showed that A2780 cells transfected with miR-199a-5p plasmid had lower migratory ability than the control cells in hypoxia and normoxia condition (Figure 2A-C).