Determination of homoarginine, arginine, NMMA, ADMA, and SDMA in biological samples by HPLC-ESI-mass spectrometry

N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-methyl-L-arginine (NMMA) are endogenous inhibitors of nitric oxide synthase (NOS). In contrast, N(G),N'(G)-dimethyl-L-arginine (SDMA) possesses only a weak inhibitory potency towards neuronal NOS and it is known to limit nitric oxide (NO) production...

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Bibliographic Details
Published in:International journal of molecular sciences 2013-10, Vol.14 (10), p.20131-20138
Main Authors: Servillo, Luigi, Giovane, Alfonso, D'Onofrio, Nunzia, Casale, Rosario, Cautela, Domenico, Castaldo, Domenico, Balestrieri, Maria Luisa
Format: Article
Language:English
Subjects:
ADMA
arginine
Arginine - analogs & derivatives
Arginine - chemistry
Cardiovascular disease
Chromatography
Chromatography, High Pressure Liquid - methods
Competition
homoarginine
Homoarginine - chemistry
Humans
Mass spectrometry
Metabolites
Nitric oxide
NMMA
Particle size
Proteins
Scientific imaging
SDMA
Tandem Mass Spectrometry - methods
Technical Note
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Summary:N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-methyl-L-arginine (NMMA) are endogenous inhibitors of nitric oxide synthase (NOS). In contrast, N(G),N'(G)-dimethyl-L-arginine (SDMA) possesses only a weak inhibitory potency towards neuronal NOS and it is known to limit nitric oxide (NO) production by competing with L-arginine for cellular uptake. The inhibition of NOS is associated with endothelial dysfunction in cardiovascular diseases as well in chronic renal failure. L-homoarginine (HArg), a structural analog of L-arginine (Arg), is an alternative but less efficient substrate for NOS. Besides, it inhibits arginase, leading to an increased availability of L-arginine for NOS to produce NO. However, its relation with cardiovascular disease remains unclear. To date, several analytical methods for the quantitative determination of Arg, HArg, NMMA, AMDA, and SDMA in biological samples have been described. Here, we present a simple, fast, and accurate HPLC-ESI-MS/MS method which allows both the simultaneous determination and quantification of these compounds without needing derivatization, and the possibility to easily modulate the chromatographic separation between HArg and NMMA (or between SDMA and ADMA). Data on biological samples revealed the feasibility of the method, the minimal sample preparation, and the fast run time which make this method very suitable and accurate for analysis in the basic and clinical settings.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms141020131