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Single-cell transcriptomics by clinical course of Mycobacterium avium complex pulmonary disease
Mycobacterium avium complex pulmonary disease (MAC-PD) has a heterogeneous clinical course. However, immune profiles associated with MAC-PD clinical course are limited. We performed single-cell RNA sequencing of peripheral blood mononuclear cells from 21 MAC-PD patients divided into three clinical c...
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| Published in: | Scientific reports 2024-07, Vol.14 (1), p.15663-12, Article 15663 |
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| creator | Kim, Su-Young Zo, Sungmin Kim, Dae Hun Shin, Sung Jae Jhun, Byung Woo |
| description | Mycobacterium avium
complex pulmonary disease (MAC-PD) has a heterogeneous clinical course. However, immune profiles associated with MAC-PD clinical course are limited. We performed single-cell RNA sequencing of peripheral blood mononuclear cells from 21 MAC-PD patients divided into three clinical courses: group A, spontaneous culture conversion; group B, stable disease without antibiotic treatment; and group C, progressive disease with antibiotic treatment. A lower proportion of NK cells and higher proportion of monocytes were noted in group C compared to combined groups A and B. The proportion of classical monocytes was higher in group C compared to groups A and B, while the proportion of non-classical monocytes decreased. EGR1, HSPA1A, HSPA1B, and CD83 were up-regulated in spontaneous culture conversion group A compared to progressive disease group C. Up-regulation of MYOM2 and LILRA4 and down-regulation of MT-ATP8, CD83, and CCL3L1 was found in progressive disease group C. PCBP1, FOS, RGCC, S100B, G0S2, AREG, and LYN were highly expressed in favorable treatment response compared to unfavorable response. Our findings may offer a comprehensive understanding of the host immune profiles that influence a particular MAC-PD clinical course and could suggest an immunological mechanism associated with the disease progression of MAC-PD. |
| doi_str_mv | 10.1038/s41598-024-66523-x |
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complex pulmonary disease (MAC-PD) has a heterogeneous clinical course. However, immune profiles associated with MAC-PD clinical course are limited. We performed single-cell RNA sequencing of peripheral blood mononuclear cells from 21 MAC-PD patients divided into three clinical courses: group A, spontaneous culture conversion; group B, stable disease without antibiotic treatment; and group C, progressive disease with antibiotic treatment. A lower proportion of NK cells and higher proportion of monocytes were noted in group C compared to combined groups A and B. The proportion of classical monocytes was higher in group C compared to groups A and B, while the proportion of non-classical monocytes decreased. EGR1, HSPA1A, HSPA1B, and CD83 were up-regulated in spontaneous culture conversion group A compared to progressive disease group C. Up-regulation of MYOM2 and LILRA4 and down-regulation of MT-ATP8, CD83, and CCL3L1 was found in progressive disease group C. PCBP1, FOS, RGCC, S100B, G0S2, AREG, and LYN were highly expressed in favorable treatment response compared to unfavorable response. Our findings may offer a comprehensive understanding of the host immune profiles that influence a particular MAC-PD clinical course and could suggest an immunological mechanism associated with the disease progression of MAC-PD.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-024-66523-x</identifier><identifier>PMID: 38977917</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>631/250/255/1318 ; 692/699/255/1318 ; Aged ; Antibiotics ; CD83 antigen ; Cell culture ; Disease Progression ; EGR-1 protein ; Female ; Gene Expression Profiling ; Humanities and Social Sciences ; Humans ; Immune profile ; Leukocytes (mononuclear) ; Leukocytes, Mononuclear - metabolism ; Leukocytes, Mononuclear - microbiology ; Lung diseases ; Lung Diseases - genetics ; Lung Diseases - microbiology ; Male ; Middle Aged ; Monocytes ; Monocytes - immunology ; Monocytes - metabolism ; multidisciplinary ; Mycobacterium avium ; Mycobacterium avium Complex - genetics ; Mycobacterium avium complex pulmonary disease ; Mycobacterium avium-intracellulare Infection - microbiology ; Peripheral blood mononuclear cells ; S100b protein ; Science ; Science (multidisciplinary) ; Single-Cell Analysis - methods ; Single-cell RNA sequencing ; Transcriptome ; Transcriptomics ; Treatment outcome</subject><ispartof>Scientific reports, 2024-07, Vol.14 (1), p.15663-12, Article 15663</ispartof><rights>The Author(s) 2024</rights><rights>2024. The Author(s).</rights><rights>The Author(s) 2024. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c422t-124344fb754cdec8a34e9412027827e493b39ce4708fe03687e9de763f2126413</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/3076842491/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/3076842491?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25753,27924,27925,37012,37013,38516,43895,44590,53791,53793,74412,75126</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38977917$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, Su-Young</creatorcontrib><creatorcontrib>Zo, Sungmin</creatorcontrib><creatorcontrib>Kim, Dae Hun</creatorcontrib><creatorcontrib>Shin, Sung Jae</creatorcontrib><creatorcontrib>Jhun, Byung Woo</creatorcontrib><title>Single-cell transcriptomics by clinical course of Mycobacterium avium complex pulmonary disease</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>Mycobacterium avium
complex pulmonary disease (MAC-PD) has a heterogeneous clinical course. However, immune profiles associated with MAC-PD clinical course are limited. We performed single-cell RNA sequencing of peripheral blood mononuclear cells from 21 MAC-PD patients divided into three clinical courses: group A, spontaneous culture conversion; group B, stable disease without antibiotic treatment; and group C, progressive disease with antibiotic treatment. A lower proportion of NK cells and higher proportion of monocytes were noted in group C compared to combined groups A and B. The proportion of classical monocytes was higher in group C compared to groups A and B, while the proportion of non-classical monocytes decreased. EGR1, HSPA1A, HSPA1B, and CD83 were up-regulated in spontaneous culture conversion group A compared to progressive disease group C. Up-regulation of MYOM2 and LILRA4 and down-regulation of MT-ATP8, CD83, and CCL3L1 was found in progressive disease group C. PCBP1, FOS, RGCC, S100B, G0S2, AREG, and LYN were highly expressed in favorable treatment response compared to unfavorable response. Our findings may offer a comprehensive understanding of the host immune profiles that influence a particular MAC-PD clinical course and could suggest an immunological mechanism associated with the disease progression of MAC-PD.</description><subject>631/250/255/1318</subject><subject>692/699/255/1318</subject><subject>Aged</subject><subject>Antibiotics</subject><subject>CD83 antigen</subject><subject>Cell culture</subject><subject>Disease Progression</subject><subject>EGR-1 protein</subject><subject>Female</subject><subject>Gene Expression Profiling</subject><subject>Humanities and Social Sciences</subject><subject>Humans</subject><subject>Immune profile</subject><subject>Leukocytes (mononuclear)</subject><subject>Leukocytes, Mononuclear - metabolism</subject><subject>Leukocytes, Mononuclear - microbiology</subject><subject>Lung diseases</subject><subject>Lung Diseases - genetics</subject><subject>Lung Diseases - microbiology</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Monocytes</subject><subject>Monocytes - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>Directory of Open Access Journals (DOAJ)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Su-Young</au><au>Zo, Sungmin</au><au>Kim, Dae Hun</au><au>Shin, Sung Jae</au><au>Jhun, Byung Woo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Single-cell transcriptomics by clinical course of Mycobacterium avium complex pulmonary disease</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2024-07-08</date><risdate>2024</risdate><volume>14</volume><issue>1</issue><spage>15663</spage><epage>12</epage><pages>15663-12</pages><artnum>15663</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>Mycobacterium avium
complex pulmonary disease (MAC-PD) has a heterogeneous clinical course. However, immune profiles associated with MAC-PD clinical course are limited. We performed single-cell RNA sequencing of peripheral blood mononuclear cells from 21 MAC-PD patients divided into three clinical courses: group A, spontaneous culture conversion; group B, stable disease without antibiotic treatment; and group C, progressive disease with antibiotic treatment. A lower proportion of NK cells and higher proportion of monocytes were noted in group C compared to combined groups A and B. The proportion of classical monocytes was higher in group C compared to groups A and B, while the proportion of non-classical monocytes decreased. EGR1, HSPA1A, HSPA1B, and CD83 were up-regulated in spontaneous culture conversion group A compared to progressive disease group C. Up-regulation of MYOM2 and LILRA4 and down-regulation of MT-ATP8, CD83, and CCL3L1 was found in progressive disease group C. PCBP1, FOS, RGCC, S100B, G0S2, AREG, and LYN were highly expressed in favorable treatment response compared to unfavorable response. Our findings may offer a comprehensive understanding of the host immune profiles that influence a particular MAC-PD clinical course and could suggest an immunological mechanism associated with the disease progression of MAC-PD.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>38977917</pmid><doi>10.1038/s41598-024-66523-x</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 631/250/255/1318 692/699/255/1318 Aged Antibiotics CD83 antigen Cell culture Disease Progression EGR-1 protein Female Gene Expression Profiling Humanities and Social Sciences Humans Immune profile Leukocytes (mononuclear) Leukocytes, Mononuclear - metabolism Leukocytes, Mononuclear - microbiology Lung diseases Lung Diseases - genetics Lung Diseases - microbiology Male Middle Aged Monocytes Monocytes - immunology Monocytes - metabolism multidisciplinary Mycobacterium avium Mycobacterium avium Complex - genetics Mycobacterium avium complex pulmonary disease Mycobacterium avium-intracellulare Infection - microbiology Peripheral blood mononuclear cells S100b protein Science Science (multidisciplinary) Single-Cell Analysis - methods Single-cell RNA sequencing Transcriptome Transcriptomics Treatment outcome |
| title | Single-cell transcriptomics by clinical course of Mycobacterium avium complex pulmonary disease |
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