Overexpression of long non-coding RNA SOX2OT promotes esophageal squamous cell carcinoma growth

SOX2 overlapping transcript (SOX2OT) has been reported to be an important lncRNA in various cancers. SOX2 is embedded in an intron of the SOX2OT gene. But the role of SOX2OT in esophageal squamous cell carcinoma (ESCC) and the association between SOX2OT and SOX2 remain unclear. Quantitative PCR (qPC...

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Published in:Cancer cell international 2018-05, Vol.18 (1), p.76-76, Article 76
Main Authors: Wu, Yuanyuan, Chen, Xuedan, Liang, Yan, Li, Juan, Zhang, Kun, Dai, Limeng, Guan, Xingying, Wang, Kai, Bai, Yun
Format: Article
Language:English
Subjects:
Breast cancer
Cell culture
Cell growth
Cell proliferation
Cholecystokinin
Cloning
Deoxyribonucleic acid
DNA
DNA methylation
Ectopic expression
ESCC
Esophageal cancer
Esophagus
Isoforms
LncRNA
Medical prognosis
Metastasis
Non-coding RNA
Primary Research
RNA polymerase
SOX2OT and SOX2
Squamous cell carcinoma
Studies
Tissues
Transcription
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cited_by cdi_FETCH-LOGICAL-c594t-f534ff3a80ff5a9e0d2fc35d992b35213a0179c50a50f3d8061d18b7843795f53
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container_title Cancer cell international
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Bai, Yun
description SOX2 overlapping transcript (SOX2OT) has been reported to be an important lncRNA in various cancers. SOX2 is embedded in an intron of the SOX2OT gene. But the role of SOX2OT in esophageal squamous cell carcinoma (ESCC) and the association between SOX2OT and SOX2 remain unclear. Quantitative PCR (qPCR) was used to detect the expression of SOX2OT and SOX2 in ESCC tissues and cells. The isoforms of SOX2OT were identified by PCR and confirmed by sequencing. CCK-8 and Edu assays were performed to investigate the effects of SOX2OT on cell growth. The relationship between SOX2OT and SOX2 was explored by luciferase reporter assay. Both SOX2OT and SOX2 were upregulated in ESCC tissues and cells. SOX2OT expression was positively associated with SOX2 expression in ESCC tissues. NR_004053 was one of the major SOX2OT transcripts aberrantly expressed in ESCC tissues and cells. Overexpression of SOX2OT (NR_004053) promoted ESCC cell growth, antagonized the effect of DDP and increased cell proliferation ratio. Ectopic expression of SOX2 could increase the luciferase activity of SOX2OT-pGL3/Basic and SOX2OT expression, while overexpression of SOX2OT (NR_004053) had no effect on SOX2 expression. Our study demonstrates that the major isoform of SOX2OT in ESCC, SOX2OT (NR_004053) contributes to cell growth. SOX2 promotes SOX2OT expression at transcriptional level.
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SOX2 is embedded in an intron of the SOX2OT gene. But the role of SOX2OT in esophageal squamous cell carcinoma (ESCC) and the association between SOX2OT and SOX2 remain unclear. Quantitative PCR (qPCR) was used to detect the expression of SOX2OT and SOX2 in ESCC tissues and cells. The isoforms of SOX2OT were identified by PCR and confirmed by sequencing. CCK-8 and Edu assays were performed to investigate the effects of SOX2OT on cell growth. The relationship between SOX2OT and SOX2 was explored by luciferase reporter assay. Both SOX2OT and SOX2 were upregulated in ESCC tissues and cells. SOX2OT expression was positively associated with SOX2 expression in ESCC tissues. NR_004053 was one of the major SOX2OT transcripts aberrantly expressed in ESCC tissues and cells. Overexpression of SOX2OT (NR_004053) promoted ESCC cell growth, antagonized the effect of DDP and increased cell proliferation ratio. Ectopic expression of SOX2 could increase the luciferase activity of SOX2OT-pGL3/Basic and SOX2OT expression, while overexpression of SOX2OT (NR_004053) had no effect on SOX2 expression. Our study demonstrates that the major isoform of SOX2OT in ESCC, SOX2OT (NR_004053) contributes to cell growth. SOX2 promotes SOX2OT expression at transcriptional level.</abstract><cop>England</cop><pub>BioMed Central</pub><pmid>29849506</pmid><doi>10.1186/s12935-018-0570-7</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record>
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subjects Breast cancer
Cell culture
Cell growth
Cell proliferation
Cholecystokinin
Cloning
Deoxyribonucleic acid
DNA
DNA methylation
Ectopic expression
ESCC
Esophageal cancer
Esophagus
Isoforms
LncRNA
Medical prognosis
Metastasis
Non-coding RNA
Primary Research
RNA polymerase
SOX2OT and SOX2
Squamous cell carcinoma
Studies
Tissues
Transcription
title Overexpression of long non-coding RNA SOX2OT promotes esophageal squamous cell carcinoma growth
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