A Rapid Capillary-Pressure Driven Micro-Channel to Demonstrate Newtonian Fluid Behavior of Zebrafish Blood at High Shear Rates

Blood viscosity provides the rheological basis to elucidate shear stress underlying developmental cardiac mechanics and physiology. Zebrafish is a high throughput model for developmental biology, forward-genetics, and drug discovery. The micro-scale posed an experimental challenge to measure blood v...

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Bibliographic Details
Published in:Scientific reports 2017-05, Vol.7 (1), p.1980-8, Article 1980
Main Authors: Lee, Juhyun, Chou, Tzu-Chieh, Kang, Dongyang, Kang, Hanul, Chen, Junjie, Baek, Kyung In, Wang, Wei, Ding, Yichen, Carlo, Dino Di, Tai, Yu-Chong, Hsiai, Tzung K.
Format: Article
Language:English
Subjects:
631/601/1737
639/166/985
Animals
Biomechanical Phenomena
Blood
Blood Viscosity
Danio rerio
Developmental biology
Drug discovery
Embryogenesis
Embryonic growth stage
Genetics
Heart diseases
Hemodynamics
Hemorheology
Humanities and Social Sciences
Mechanical stimuli
Microfluidics
Microfluidics - methods
multidisciplinary
Pressure
Reproducibility of Results
Science
Science (multidisciplinary)
Shear stress
Stress, Mechanical
Surface tension
Vacuum
Viscosity
Zebrafish
Zebrafish - physiology
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Summary:Blood viscosity provides the rheological basis to elucidate shear stress underlying developmental cardiac mechanics and physiology. Zebrafish is a high throughput model for developmental biology, forward-genetics, and drug discovery. The micro-scale posed an experimental challenge to measure blood viscosity. To address this challenge, a microfluidic viscometer driven by surface tension was developed to reduce the sample volume required (3μL) for rapid (500 s −1 ), at which the power law exponent (n) of zebrafish blood was nearly 1 behaving as a Newtonian fluid. The measured values of whole blood from the micro-channel (4.17cP) and the vacuum method (4.22cP) at 500 s −1 were closely correlated at 27 °C. A calibration curve was established for viscosity as a function of hematocrits to predict a rise and fall in viscosity during embryonic development. Thus, our rapid capillary pressure-driven micro-channel revealed the Newtonian fluid behavior of zebrafish blood at high shear rates and the dynamic viscosity during development.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-017-02253-7