Highly Sensitive Fluorescence Detection of Three Organophosphorus Pesticides Based on Highly Bright DNA-Templated Silver Nanoclusters

It is still challenging to achieve simultaneous and sensitive detection of multiple organophosphorus pesticides (OPs). Herein, we optimized the ssDNA templates for the synthesis of silver nanoclusters (Ag NCs). For the first time, we found that the fluorescence intensity of T base-extended DNA-templ...

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Bibliographic Details
Published in:Biosensors (Basel) 2023-05, Vol.13 (5), p.520
Main Authors: Li, Guowen, Huang, Xiufang, Peng, Chifang, Sun, Fengxia
Format: Article
Language:English
Subjects:
Bonding strength
Caustic soda
Deoxyribonucleic acid
Dimethoate
DNA
DNA - chemistry
DNA-templated silver nanoclusters
Enzymes
Fluorescence
fluorescence quenching
fluorescence sensor
Hydrolysates
Lakes
Metal Nanoparticles - chemistry
Methods
Nanoclusters
Nanoparticles
Organophosphorus Compounds
Organophosphorus pesticides
Pesticides
Phorate
Quantum dots
Silver
Silver - chemistry
Spectrometry, Fluorescence
Sulfhydryl groups
Water analysis
Water sampling
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Summary:It is still challenging to achieve simultaneous and sensitive detection of multiple organophosphorus pesticides (OPs). Herein, we optimized the ssDNA templates for the synthesis of silver nanoclusters (Ag NCs). For the first time, we found that the fluorescence intensity of T base-extended DNA-templated Ag NCs was over three times higher than the original C-riched DNA-templated Ag NCs. Moreover, a "turn-off" fluorescence sensor based on the brightest DNA-Ag NCs was constructed for the sensitive detection of dimethoate, ethion and phorate. Under strong alkaline conditions, the P-S bonds in three pesticides were broken, and the corresponding hydrolysates were obtained. The sulfhydryl groups in the hydrolyzed products formed Ag-S bonds with the silver atoms on the surface of Ag NCs, which resulted in the aggregation of Ag NCs, following the fluorescence quenching. The fluorescence sensor showed that the linear ranges were 0.1-4 ng/mL for dimethoate with a limit of detection (LOD) of 0.05 ng/mL, 0.3-2 µg/mL for ethion with a LOD of 30 ng/mL, and 0.03-0.25 µg/mL for phorate with a LOD of 3 ng/mL. Moreover, the developed method was successfully applied to the detection of dimethoate, ethion and phorate in lake water samples, indicating a potential application in OP detection.
ISSN:2079-6374
2079-6374
DOI:10.3390/bios13050520