Detection of monohydroxy "bile" acids in the brains of guinea pigs afflicted with experimental allergic encephalomyelitis

3alpha-Hydroxy-5-cholanoic acid (lithocholic acid) and some unidentified acids (one of them perhaps 3-hydroxy-cholest-5-en-26-oic acid) have been detected in the brains of guinea pigs afflicted with experimental "allergic" encephalomyelitis. None of the acids could be identified in compara...

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Bibliographic Details
Published in:Journal of lipid research 1969-01, Vol.10 (1), p.115-120
Main Authors: Naqvi, S H, Herndon, B L, Kelley, M T, Bleisch, V, Aexel, R T, Nicholas, H J
Format: Article
Language:English
Subjects:
Animals
Bile Acids and Salts - metabolism
Brain Chemistry
Carbon Isotopes
Cholesterol - metabolism
cholesterol oxidation
Chromatography, Gas
Chromatography, Thin Layer
demyelination
Encephalomyelitis, Autoimmune, Experimental - metabolism
Esters - analysis
Fatty Acids - analysis
gas-liquid chromatography
Guinea Pigs
lithocholic acid
Male
mass spectrometry
Spectrum Analysis
thin-layer chromatography
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Summary:3alpha-Hydroxy-5-cholanoic acid (lithocholic acid) and some unidentified acids (one of them perhaps 3-hydroxy-cholest-5-en-26-oic acid) have been detected in the brains of guinea pigs afflicted with experimental "allergic" encephalomyelitis. None of the acids could be identified in comparable amounts of brain tissue from normal guinea pigs. Thin-layer chromatography of the free acids, and thin-layer and gas-liquid chromatography combined with mass spectrometry of the methyl esters were used for identification. The occurrence of these acids may possibly be the result of cholesterol oxidation in the brain. The acids, or compounds related to them, may play a role in development of demyelination in experimental allergic encephalomyelitis.
ISSN:0022-2275
DOI:10.1016/S0022-2275(20)42655-0