Extraction, Isolation and Identification of Low Molecular Weight Peptides in Human Milk

Human milk contains numerous free low molecular weight peptides (LMWPs), which may play an important role in infant health and growth. The bioactivities of LMWPs are determined by their structures, especially the amino acid sequences. In the present study, 81 human milk samples were collected and pu...

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Bibliographic Details
Published in:Foods 2022-06, Vol.11 (13), p.1836
Main Authors: Xiao, Hailong, Jiang, He, Tu, Haiyun, Jia, Yanbo, Wang, Hongqing, Lü, Xin, Fang, Ruosi, Xiao, Gongnian
Format: Article
Language:English
Subjects:
Amino acids
Breast milk
Breastfeeding & lactation
Casein
Cation exchanging
Chromatography
Food science
free low molecular weight peptides
High performance liquid chromatography
human milk
identification
Liquid chromatography
Low molecular weights
Mass spectrometry
Mass spectroscopy
Milk
Molecular weight
Peptides
Proteins
Quadrupoles
Scientific imaging
Software
Solid phases
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Summary:Human milk contains numerous free low molecular weight peptides (LMWPs), which may play an important role in infant health and growth. The bioactivities of LMWPs are determined by their structures, especially the amino acid sequences. In the present study, 81 human milk samples were collected and purified by cation-exchange solid-phase extraction (SPE). Ultra-high performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS) was used for the separation and detection of free LMWPs in human milk. A total of 56 LMWPs were identified and quantified. These LMWPs were mainly derived from 3 regions of β-casein, which were the amino acid fragments of 16–40, 85–110, and 205–226. The predominant LMWPs were RETIESLSSSEESITEYK, RETIESLSSSEESITEYKQKVEKVK, ETIESLSSSEESITEYK, TQPLAPVHNPIS, and QPLAPVHNPISV with molecular weights of 2247.9573, 2860.2437, 2091.8591, 1372.7666, and 1271.7212, respectively. The results indicated that the technique based on SPE and UPLC-QTOF-MS might greatly facilitate the analysis of LMWPs in human milk.
ISSN:2304-8158
2304-8158
DOI:10.3390/foods11131836