Solid-Phase Synthesis of Red Fluorescent Carbon Dots for the Dual-Mode Detection of Hexavalent Chromium and Cell Imaging

The excellent optical properties and biocompatibility of red fluorescence carbon dots (R-CDs) provide a new approach for the effective analysis of hexavalent chromium Cr(VI) in environmental and biological samples. However, the application of R-CDs is still limited by low yield and unfriendly synthe...

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Bibliographic Details
Published in:Biosensors (Basel) 2022-06, Vol.12 (6), p.432
Main Authors: Hu, Jinshuang, Wang, Xin, Wei, Hua, Zhao, Lei, Yao, Boxuan, Zhang, Caiyun, Zhou, Jiarui, Liu, Jian, Yang, Shenghong
Format: Article
Language:English
Subjects:
Absorption
Aqueous solutions
Biocompatibility
Biological properties
Biological samples
Carbon
Carbon dots
Chemical detectors
Chemical properties
Chemical synthesis
Chromium
Cr(VI) detection
Design and construction
Drinking water
Electromagnetic absorption
Environmental aspects
Environmental protection
Fluorescence
Fourier transforms
Hexavalent chromium
Hydrochloric acid
Measurement
Methods
Optical properties
Optics
Production processes
Quantum dots
R-CDs
Raw materials
scanometric and fluorescent
Scientific imaging
Solid phase methods
Solid phase synthesis
Solvents
Stainless steel
Water analysis
Water sampling
Wood preservatives
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Summary:The excellent optical properties and biocompatibility of red fluorescence carbon dots (R-CDs) provide a new approach for the effective analysis of hexavalent chromium Cr(VI) in environmental and biological samples. However, the application of R-CDs is still limited by low yield and unfriendly synthesis route. In this study, we developed a new type of R-CDs based on a simple and green solid-phase preparation strategy. The synthesized R-CDs can emit bright red fluorescence with an emission wavelength of 625 nm and also have an obvious visible light absorption capacity. Furthermore, the absorption and fluorescence signals of the R-CDs aqueous solution are sensitive to Cr(VI), which is reflected in color change and fluorescence quenching. Based on that, a scanometric and fluorescent dual-mode analysis system for the rapid and accurate detection of Cr(VI) was established well within the limit of detection at 80 nM and 9.1 nM, respectively. The proposed methods also possess high specificity and were applied for the detection of Cr(VI) in real water samples. More importantly, the synthesized R-CDs with good biocompatibility were further successfully applied for visualizing intracellular Cr(VI) in Hela cells.
ISSN:2079-6374
2079-6374
DOI:10.3390/bios12060432