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LncRNA XIST promotes liver cancer progression by acting as a molecular sponge of miR-200b-3p to regulate ZEB1/2 expression
Objective To evaluate the predictive value of long non-coding RNA (lncRNA) X-inactive specific transcript (XIST) for survival, and determine the involvement of miRNA(miR)-200b-3p and zinc finger E-box-binding homeobox (ZEB) 1/2 in the pro-tumor effect of lncRNA XIST in liver cancer. Methods We evalu...
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| Published in: | Journal of international medical research 2021-05, Vol.49 (5), p.3000605211016211-3000605211016211 |
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| Language: | English |
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| container_end_page | 3000605211016211 |
| container_issue | 5 |
| container_start_page | 3000605211016211 |
| container_title | Journal of international medical research |
| container_volume | 49 |
| creator | Liu, Lili Jiang, Hua Pan, Hongming Zhu, Xiuming |
| description | Objective
To evaluate the predictive value of long non-coding RNA (lncRNA) X-inactive specific transcript (XIST) for survival, and determine the involvement of miRNA(miR)-200b-3p and zinc finger E-box-binding homeobox (ZEB) 1/2 in the pro-tumor effect of lncRNA XIST in liver cancer.
Methods
We evaluated lncRNA XIST expression in liver cancer tissues and cell lines by quantitative reverse transcription polymerase chain reaction (RT-qPCR) and analyzed the correlation between its expression and overall survival of liver cancer patients by Kaplan–Meier analysis. Its effects on cell proliferation, migration, and invasion were analyzed by Cell-Counting Kit-8 and Transwell assays. The association between lncRNA XIST and miR-200b-3p, and the effects of lncRNA XIST on ZEB1/2 expression were explored using luciferase reporter assays, real-time PCR, and western blotting.
Results
The lncRNA XIST was significantly upregulated in liver cancer, and increased lncRNA XIST expression was associated with a poor prognosis. The lncRNA XIST promoted liver cancer cell proliferation, migration, and invasion in vitro, and acted as a molecular sponge for miR-200b-3p, and also regulated the expression of ZEB1/2 via miR-200b-3p.
Conclusion
The lncRNA XIST is an oncogenic lncRNA that promotes liver cancer metastasis, and its pro-metastatic phenotype can be partially attributed to the lncRNA XIST/miR-200b-3p/ZEB1/2 signaling axis. |
| doi_str_mv | 10.1177/03000605211016211 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_doaj_</sourceid><recordid>TN_cdi_doaj_primary_oai_doaj_org_article_44e14eea87814139922a63456cd9fe07</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sage_id>10.1177_03000605211016211</sage_id><doaj_id>oai_doaj_org_article_44e14eea87814139922a63456cd9fe07</doaj_id><sourcerecordid>2531222665</sourcerecordid><originalsourceid>FETCH-LOGICAL-c532t-23bee2c243b916f722ade1a71bd29e1675bad08f4052370bd668e665f9a5408a3</originalsourceid><addsrcrecordid>eNp1kk1v1DAQQC0EokvhB3BBlrhwSTv-THJBKlWBlVYglSIhLpbjTEJWSRzsbEX59TjsUiiIi0fyvHn2jIaQpwxOGMvzUxAAoEFxxoDpdN4jKyZzkfGUuE9WSz5bgCPyKMYtgORa8YfkSEhgRSFhRb5vRnf57ox-Wn-4olPwg58x0r67xkCdHV0K6bYNGGPnR1rdUOvmbmypjdTSwffodr0NNE5-bJH6hg7dZcYBqkxMdPY0YJuAGenni1fslFP8Nh1kj8mDxvYRnxziMfn4-uLq_G22ef9mfX62yZwSfE69VIjccSmqkukm59zWyGzOqpqXyHSuKltD0cg0B5FDVWtdoNaqKa2SUFhxTNZ7b-3t1kyhG2y4Md525ueFD62xYe5cj0ZKZBLRFnnBJBNlmR7TQirt6rJByJPr5d417aoBa4fjHGx_R3o3M3ZfTOuvTfJxJRbBi4Mg-K87jLMZuuiw7-2IfhdNghjnPP0_oc__Qrd-F8Y0qoVSIJQoFyHbUy74GAM2t59hYJYtMf9sSap59mcXtxW_1iIBJ3sg2hZ_P_t_4w99DMIO</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2535035397</pqid></control><display><type>article</type><title>LncRNA XIST promotes liver cancer progression by acting as a molecular sponge of miR-200b-3p to regulate ZEB1/2 expression</title><source>NCBI_PubMed Central（免费）</source><source>Publicly Available Content Database</source><source>Sage Journals GOLD Open Access 2024</source><creator>Liu, Lili ; Jiang, Hua ; Pan, Hongming ; Zhu, Xiuming</creator><creatorcontrib>Liu, Lili ; Jiang, Hua ; Pan, Hongming ; Zhu, Xiuming</creatorcontrib><description>Objective
To evaluate the predictive value of long non-coding RNA (lncRNA) X-inactive specific transcript (XIST) for survival, and determine the involvement of miRNA(miR)-200b-3p and zinc finger E-box-binding homeobox (ZEB) 1/2 in the pro-tumor effect of lncRNA XIST in liver cancer.
Methods
We evaluated lncRNA XIST expression in liver cancer tissues and cell lines by quantitative reverse transcription polymerase chain reaction (RT-qPCR) and analyzed the correlation between its expression and overall survival of liver cancer patients by Kaplan–Meier analysis. Its effects on cell proliferation, migration, and invasion were analyzed by Cell-Counting Kit-8 and Transwell assays. The association between lncRNA XIST and miR-200b-3p, and the effects of lncRNA XIST on ZEB1/2 expression were explored using luciferase reporter assays, real-time PCR, and western blotting.
Results
The lncRNA XIST was significantly upregulated in liver cancer, and increased lncRNA XIST expression was associated with a poor prognosis. The lncRNA XIST promoted liver cancer cell proliferation, migration, and invasion in vitro, and acted as a molecular sponge for miR-200b-3p, and also regulated the expression of ZEB1/2 via miR-200b-3p.
Conclusion
The lncRNA XIST is an oncogenic lncRNA that promotes liver cancer metastasis, and its pro-metastatic phenotype can be partially attributed to the lncRNA XIST/miR-200b-3p/ZEB1/2 signaling axis.</description><identifier>ISSN: 0300-0605</identifier><identifier>EISSN: 1473-2300</identifier><identifier>DOI: 10.1177/03000605211016211</identifier><identifier>PMID: 34018840</identifier><language>eng</language><publisher>London, England: SAGE Publications</publisher><subject>Cell growth ; Cell Proliferation - genetics ; Humans ; Liver cancer ; Liver Neoplasms - genetics ; Medical prognosis ; MicroRNAs - genetics ; Pre-Clinical Research Report ; RNA, Long Noncoding - genetics ; Signal Transduction ; Zinc Finger E-box-Binding Homeobox 1 - genetics ; Zinc Finger E-box-Binding Homeobox 1 - metabolism</subject><ispartof>Journal of international medical research, 2021-05, Vol.49 (5), p.3000605211016211-3000605211016211</ispartof><rights>The Author(s) 2021</rights><rights>The Author(s) 2021. This work is licensed under the Creative Commons Attribution – Non-Commercial License https://creativecommons.org/licenses/by-nc/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>The Author(s) 2021 2021 SAGE Publications</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c532t-23bee2c243b916f722ade1a71bd29e1675bad08f4052370bd668e665f9a5408a3</citedby><cites>FETCH-LOGICAL-c532t-23bee2c243b916f722ade1a71bd29e1675bad08f4052370bd668e665f9a5408a3</cites><orcidid>0000-0002-7185-894X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8142537/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2535035397?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,21945,25731,27830,27901,27902,36989,36990,44566,44921,45309,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34018840$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Lili</creatorcontrib><creatorcontrib>Jiang, Hua</creatorcontrib><creatorcontrib>Pan, Hongming</creatorcontrib><creatorcontrib>Zhu, Xiuming</creatorcontrib><title>LncRNA XIST promotes liver cancer progression by acting as a molecular sponge of miR-200b-3p to regulate ZEB1/2 expression</title><title>Journal of international medical research</title><addtitle>J Int Med Res</addtitle><description>Objective
To evaluate the predictive value of long non-coding RNA (lncRNA) X-inactive specific transcript (XIST) for survival, and determine the involvement of miRNA(miR)-200b-3p and zinc finger E-box-binding homeobox (ZEB) 1/2 in the pro-tumor effect of lncRNA XIST in liver cancer.
Methods
We evaluated lncRNA XIST expression in liver cancer tissues and cell lines by quantitative reverse transcription polymerase chain reaction (RT-qPCR) and analyzed the correlation between its expression and overall survival of liver cancer patients by Kaplan–Meier analysis. Its effects on cell proliferation, migration, and invasion were analyzed by Cell-Counting Kit-8 and Transwell assays. The association between lncRNA XIST and miR-200b-3p, and the effects of lncRNA XIST on ZEB1/2 expression were explored using luciferase reporter assays, real-time PCR, and western blotting.
Results
The lncRNA XIST was significantly upregulated in liver cancer, and increased lncRNA XIST expression was associated with a poor prognosis. The lncRNA XIST promoted liver cancer cell proliferation, migration, and invasion in vitro, and acted as a molecular sponge for miR-200b-3p, and also regulated the expression of ZEB1/2 via miR-200b-3p.
Conclusion
The lncRNA XIST is an oncogenic lncRNA that promotes liver cancer metastasis, and its pro-metastatic phenotype can be partially attributed to the lncRNA XIST/miR-200b-3p/ZEB1/2 signaling axis.</description><subject>Cell growth</subject><subject>Cell Proliferation - genetics</subject><subject>Humans</subject><subject>Liver cancer</subject><subject>Liver Neoplasms - genetics</subject><subject>Medical prognosis</subject><subject>MicroRNAs - genetics</subject><subject>Pre-Clinical Research Report</subject><subject>RNA, Long Noncoding - genetics</subject><subject>Signal Transduction</subject><subject>Zinc Finger E-box-Binding Homeobox 1 - genetics</subject><subject>Zinc Finger E-box-Binding Homeobox 1 - metabolism</subject><issn>0300-0605</issn><issn>1473-2300</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>AFRWT</sourceid><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNp1kk1v1DAQQC0EokvhB3BBlrhwSTv-THJBKlWBlVYglSIhLpbjTEJWSRzsbEX59TjsUiiIi0fyvHn2jIaQpwxOGMvzUxAAoEFxxoDpdN4jKyZzkfGUuE9WSz5bgCPyKMYtgORa8YfkSEhgRSFhRb5vRnf57ox-Wn-4olPwg58x0r67xkCdHV0K6bYNGGPnR1rdUOvmbmypjdTSwffodr0NNE5-bJH6hg7dZcYBqkxMdPY0YJuAGenni1fslFP8Nh1kj8mDxvYRnxziMfn4-uLq_G22ef9mfX62yZwSfE69VIjccSmqkukm59zWyGzOqpqXyHSuKltD0cg0B5FDVWtdoNaqKa2SUFhxTNZ7b-3t1kyhG2y4Md525ueFD62xYe5cj0ZKZBLRFnnBJBNlmR7TQirt6rJByJPr5d417aoBa4fjHGx_R3o3M3ZfTOuvTfJxJRbBi4Mg-K87jLMZuuiw7-2IfhdNghjnPP0_oc__Qrd-F8Y0qoVSIJQoFyHbUy74GAM2t59hYJYtMf9sSap59mcXtxW_1iIBJ3sg2hZ_P_t_4w99DMIO</recordid><startdate>20210501</startdate><enddate>20210501</enddate><creator>Liu, Lili</creator><creator>Jiang, Hua</creator><creator>Pan, Hongming</creator><creator>Zhu, Xiuming</creator><general>SAGE Publications</general><general>Sage Publications Ltd</general><general>SAGE Publishing</general><scope>AFRWT</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-7185-894X</orcidid></search><sort><creationdate>20210501</creationdate><title>LncRNA XIST promotes liver cancer progression by acting as a molecular sponge of miR-200b-3p to regulate ZEB1/2 expression</title><author>Liu, Lili ; Jiang, Hua ; Pan, Hongming ; Zhu, Xiuming</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c532t-23bee2c243b916f722ade1a71bd29e1675bad08f4052370bd668e665f9a5408a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Cell growth</topic><topic>Cell Proliferation - genetics</topic><topic>Humans</topic><topic>Liver cancer</topic><topic>Liver Neoplasms - genetics</topic><topic>Medical prognosis</topic><topic>MicroRNAs - genetics</topic><topic>Pre-Clinical Research Report</topic><topic>RNA, Long Noncoding - genetics</topic><topic>Signal Transduction</topic><topic>Zinc Finger E-box-Binding Homeobox 1 - genetics</topic><topic>Zinc Finger E-box-Binding Homeobox 1 - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Lili</creatorcontrib><creatorcontrib>Jiang, Hua</creatorcontrib><creatorcontrib>Pan, Hongming</creatorcontrib><creatorcontrib>Zhu, Xiuming</creatorcontrib><collection>Sage Journals GOLD Open Access 2024</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health Medical collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>Directory of Open Access Journals</collection><jtitle>Journal of international medical research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Lili</au><au>Jiang, Hua</au><au>Pan, Hongming</au><au>Zhu, Xiuming</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>LncRNA XIST promotes liver cancer progression by acting as a molecular sponge of miR-200b-3p to regulate ZEB1/2 expression</atitle><jtitle>Journal of international medical research</jtitle><addtitle>J Int Med Res</addtitle><date>2021-05-01</date><risdate>2021</risdate><volume>49</volume><issue>5</issue><spage>3000605211016211</spage><epage>3000605211016211</epage><pages>3000605211016211-3000605211016211</pages><issn>0300-0605</issn><eissn>1473-2300</eissn><abstract>Objective
To evaluate the predictive value of long non-coding RNA (lncRNA) X-inactive specific transcript (XIST) for survival, and determine the involvement of miRNA(miR)-200b-3p and zinc finger E-box-binding homeobox (ZEB) 1/2 in the pro-tumor effect of lncRNA XIST in liver cancer.
Methods
We evaluated lncRNA XIST expression in liver cancer tissues and cell lines by quantitative reverse transcription polymerase chain reaction (RT-qPCR) and analyzed the correlation between its expression and overall survival of liver cancer patients by Kaplan–Meier analysis. Its effects on cell proliferation, migration, and invasion were analyzed by Cell-Counting Kit-8 and Transwell assays. The association between lncRNA XIST and miR-200b-3p, and the effects of lncRNA XIST on ZEB1/2 expression were explored using luciferase reporter assays, real-time PCR, and western blotting.
Results
The lncRNA XIST was significantly upregulated in liver cancer, and increased lncRNA XIST expression was associated with a poor prognosis. The lncRNA XIST promoted liver cancer cell proliferation, migration, and invasion in vitro, and acted as a molecular sponge for miR-200b-3p, and also regulated the expression of ZEB1/2 via miR-200b-3p.
Conclusion
The lncRNA XIST is an oncogenic lncRNA that promotes liver cancer metastasis, and its pro-metastatic phenotype can be partially attributed to the lncRNA XIST/miR-200b-3p/ZEB1/2 signaling axis.</abstract><cop>London, England</cop><pub>SAGE Publications</pub><pmid>34018840</pmid><doi>10.1177/03000605211016211</doi><orcidid>https://orcid.org/0000-0002-7185-894X</orcidid><oa>free_for_read</oa></addata></record> |
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| source | NCBI_PubMed Central（免费）; Publicly Available Content Database; Sage Journals GOLD Open Access 2024 |
| subjects | Cell growth Cell Proliferation - genetics Humans Liver cancer Liver Neoplasms - genetics Medical prognosis MicroRNAs - genetics Pre-Clinical Research Report RNA, Long Noncoding - genetics Signal Transduction Zinc Finger E-box-Binding Homeobox 1 - genetics Zinc Finger E-box-Binding Homeobox 1 - metabolism |
| title | LncRNA XIST promotes liver cancer progression by acting as a molecular sponge of miR-200b-3p to regulate ZEB1/2 expression |
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