Near-Infrared Fluorescence Imaging of EGFR-Overexpressing Tumors in the Mouse Xenograft Model Using scFv-IRDye800CW and Cetuximab-IRDye800CW

EGFR (epidermal growth factor receptor) is overexpressed in a variety of human cancers (including squamous cell carcinoma of head and neck, colon cancer, and some breast cancers) and therefore is regarded as an ideal target for cancer therapy or imaging purposes. In the current study, we produced a...

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Bibliographic Details
Published in:Molecular imaging 2022, Vol.2022, p.9589820-9589820
Main Authors: Amini, Abolfazl, Safdari, Yaghoub, Tash Shamsabadi, Fatemeh
Format: Article
Language:English
Subjects:
Animals
Cancer therapies
Cell Line, Tumor
Cetuximab
Colon cancer
Disease Models, Animal
Epidermal growth factor receptors
ErbB Receptors - metabolism
Growth factors
Head & neck cancer
Head and neck carcinoma
Heterografts
Humans
I.R. radiation
Immunoconjugates
Infrared imaging
Infrared instruments
Mice
Mice, Nude
Monoclonal antibodies
Optical Imaging - methods
Squamous cell carcinoma
Tumors
Xenotransplantation
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Summary:EGFR (epidermal growth factor receptor) is overexpressed in a variety of human cancers (including squamous cell carcinoma of head and neck, colon cancer, and some breast cancers) and therefore is regarded as an ideal target for cancer therapy or imaging purposes. In the current study, we produced a scFv-based near-infrared probe (called cet.Hum.scFv-IRDye-800CW) and evaluated its ability in recognizing and imaging of EGFR-overexpressing tumors in a mouse model. Like the molecular probe consisting of its parental antibody (cetuximab, an FDA-approved monoclonal antibody) and IRD800CW, cet.Hum.scFv-IRDye-800CW was able to recognize EGFR-overexpressing tumors in mice. cet.Hum.scFv-IRDye-800CW was found to be superior to the cetuximab-based probe in imaging of mouse tumors. The tumor-to-background ratio and blood clearance rate were higher when cet.Hum.scFv-IRDye-800CW was used as an imaging probe.
ISSN:1536-0121
1535-3508
1536-0121
DOI:10.1155/2022/9589820