Differential Contributions of mSWI/SNF Chromatin Remodeler Sub-Families to Myoblast Differentiation

Mammalian SWI/SNF (mSWI/SNF) complexes are ATP-dependent chromatin remodeling enzymes that are critical for normal cellular functions. mSWI/SNF enzymes are classified into three sub-families based on the presence of specific subunit proteins. The sub-families are Brm- or Brg1-associated factor (BAF)...

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Published in:International journal of molecular sciences 2023-07, Vol.24 (14), p.11256
Main Authors: Padilla-Benavides, Teresita, Olea-Flores, Monserrat, Sharma, Tapan, Syed, Sabriya A, Witwicka, Hanna, Zuñiga-Eulogio, Miriam D, Zhang, Kexin, Navarro-Tito, Napoleon, Imbalzano, Anthony N
Format: Article
Language:English
Subjects:
Animals
Baf250A
Brd9
Cell cycle
Chromatin
Chromatin Assembly and Disassembly - genetics
Chromosomal Proteins, Non-Histone - genetics
Chromosomal Proteins, Non-Histone - metabolism
Enzymes
Gene expression
gene regulation
Genes
Genomes
Humans
Kinases
Mammals - metabolism
Musculoskeletal system
Myoblasts - metabolism
Myogenesis
myogenin
Proteins
Roles
SWI/SNF
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Summary:Mammalian SWI/SNF (mSWI/SNF) complexes are ATP-dependent chromatin remodeling enzymes that are critical for normal cellular functions. mSWI/SNF enzymes are classified into three sub-families based on the presence of specific subunit proteins. The sub-families are Brm- or Brg1-associated factor (BAF), ncBAF (non-canonical BAF), and polybromo-associated BAF (PBAF). The biological roles for the different enzyme sub-families are poorly described. We knocked down the expression of genes encoding unique subunit proteins for each sub-family, , , and , which mark the BAF, ncBAF, and PBAF sub-families, respectively, and examined the requirement for each in myoblast differentiation. We found that Baf250A and the BAF complex were required to drive lineage-specific gene expression. KD of delayed differentiation. However, while the Baf250A-dependent gene expression profile included myogenic genes, the Brd9-dependent gene expression profile did not, suggesting Brd9 and the ncBAF complex indirectly contributed to differentiation. Baf180 was dispensable for myoblast differentiation. The results distinguish between the roles of the mSWI/SNF enzyme sub-families during myoblast differentiation.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms241411256