Comparative assessment of genomic DNA extraction processes for Plasmodium: Identifying the appropriate method

Plasmodium DNA, in addition to being used for molecular diagnosis of malaria, find utility in monitoring patient responses to antimalarial drugs, drug resistance studies, genotyping and sequencing purposes. Over the years, numerous protocols have been proposed for extracting Plasmodium DNA from a va...

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Bibliographic Details
Published in:Journal of vector borne diseases 2015-12, Vol.52 (4), p.273-280
Main Authors: Mann, Riti, Sharma, Supriya, Mishra, Neelima, Valecha, Neena, Anvikar, Anupkumar R
Format: Article
Language:English
Subjects:
Analytic Sample Preparation Methods - economics
Analytic Sample Preparation Methods - methods
Blood clots
Deoxyribonucleic acid
DNA
DNA isolation
malaria
molecular tools
Plasmodium
protocols
DNA sequencing
DNA, Protozoan - genetics
DNA, Protozoan - isolation & purification
Drug resistance
Genetic aspects
Genotype
Humans
Malaria
Malaria - parasitology
Methods
Microbial drug resistance
Microscopy
Parasites
Parasitic diseases
Plasmodium (Protozoa)
Plasmodium - genetics
Plasmodium - isolation & purification
Quality
Studies
Thermal cycling
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Summary:Plasmodium DNA, in addition to being used for molecular diagnosis of malaria, find utility in monitoring patient responses to antimalarial drugs, drug resistance studies, genotyping and sequencing purposes. Over the years, numerous protocols have been proposed for extracting Plasmodium DNA from a variety of sources. Given that DNA isolation is fundamental to successful molecular studies, here we review the most commonly used methods for Plasmodium genomic DNA isolation, emphasizing their pros and cons. A comparison of these existing methods has been made, to evaluate their appropriateness for use in different applications and identify the method suitable for a particular laboratory based study. Selection of a suitable and accessible DNA extraction method for Plasmodium requires consideration of many factors, the most important being sensitivity, cost-effectiveness and, purity and stability of isolated DNA. Need of the hour is to accentuate on the development of a method that upholds well on all these parameters.
ISSN:0972-9062
DOI:10.4103/0972-9062.172804