miRNA signatures can predict acute liver failure in hepatitis E infected pregnant females

Acute viral hepatitis E (AVH-E) can often result in acute liver failure (ALF) during pregnancy. microRNAs serve as mediators in drug induced liver failure. We investigated their role as a biomarker in predicting ALF due to HEV (ALF-E). We performed next generation sequencing and subsequent validatio...

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Bibliographic Details
Published in:Heliyon 2017-04, Vol.3 (4), p.e00287-e00287, Article e00287
Main Authors: Trehanpati, Nirupma, Sehgal, Rashi, Patra, Sharda, Vyas, Ashish, Vasudevan, Madavan, Khosla, Ritu, Khanam, Arshi, Kumar, Guresh, Maiwall, Rakhi, Ramakrishna, Gayatri, Kottilil, Shyam, Sarin, Shiv Kumar
Format: Article
Language:English
Subjects:
biomarkers
cell proliferation
death
drugs
Health sciences
hepatitis E
liver failure
microRNA
pregnancy
therapeutics
Virology
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Summary:Acute viral hepatitis E (AVH-E) can often result in acute liver failure (ALF) during pregnancy. microRNAs serve as mediators in drug induced liver failure. We investigated their role as a biomarker in predicting ALF due to HEV (ALF-E). We performed next generation sequencing and subsequent validation studies in PBMCs of pregnant (P) self limiting AVH-E, ALF due to HEV (ALF-E) and compared with AVH-E in non-pregnant (NP) females and healthy controls. Eleven microRNAs were significantly expressed in response to HEV infection; importantly, miR- 431, 654, 1468 and 4435, were distinctly expressed in pregnant self-limiting AVH-E and healthy females (p = 0.0005), but not in ALF-E. Sixteen exclusive microRNAs differentiated ALF-E from self limiting AVH-E in pregnant females. miR-450b which affects cellular proliferation and metabolic processes through RNF20 and SECB was predominanlty upregulated and correlated with poor outcome (ROC 0.958, p = 0.001). Our results reveal that a specific microRNA profile can predict fatality in ALF-E in pregnancy. These microRNAs could be exploited as prognostic biomarkers and help in the development of new therapeutic interventions.
ISSN:2405-8440
2405-8440
DOI:10.1016/j.heliyon.2017.e00287