Qualitative and Quantitative Phytochemical Analysis of Different Extracts from Thymus algeriensis Aerial Parts

This study was performed to evaluate the metabolite recovery from different extraction methods applied to aerial parts. A high-performance liquid chromatographic method using photodiode array detector with gradient elution has been developed and validated for the simultaneous estimation of different...

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Bibliographic Details
Published in:Molecules (Basel, Switzerland) Switzerland), 2018-02, Vol.23 (2), p.463
Main Authors: Boutaoui, Nassima, Zaiter, Lahcene, Benayache, Fadila, Benayache, Samir, Carradori, Simone, Cesa, Stefania, Giusti, Anna Maria, Campestre, Cristina, Menghini, Luigi, Innosa, Denise, Locatelli, Marcello
Format: Article
Language:English
Subjects:
Acids
Carotenoids
Chlorophyll
Chromatography
color analysis
Elution
Flavonoids
HPLC-PDA
MAE
Particle size
Phenolic compounds
Phenols
Phytochemicals
pigments
Pore size
Qualitative analysis
Qualitative research
SFE
Solvents
Thymus
Thymus algeriensis
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Summary:This study was performed to evaluate the metabolite recovery from different extraction methods applied to aerial parts. A high-performance liquid chromatographic method using photodiode array detector with gradient elution has been developed and validated for the simultaneous estimation of different phenolic compounds in the extracts and in their corresponding purified fractions. The experimental results show that microwave-assisted aqueous extraction for 15 min at 100 °C gave the most phenolics-enriched extract, reducing extraction time without degradation effects on bioactives. Sixteen compounds were identified in this extract, 11 phenolic compounds and five flavonoids, all known for their biological activities. Color analysis and determination of chlorophylls and carotenoids implemented the knowledge of the chemical profile of this plant.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules23020463