Total Triterpenoid Extraction from Inonotus Obliquus Using Ionic Liquids and Separation of Potential Lactate Dehydrogenase Inhibitors via Ultrafiltration High-Speed Countercurrent Chromatography

Extracts of the fungus exhibit cytotoxic properties against different cancers; hence, this fungal species has been extensively studied. This study aimed to extract total triterpenoids from using ionic liquids (ILs) and separate potential lactate dehydrogenase (LDH) inhibitors via ultrafiltration (UF...

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Bibliographic Details
Published in:Molecules (Basel, Switzerland) Switzerland), 2021-04, Vol.26 (9), p.2467
Main Authors: Wang, Yueqi, Guo, Liping, Liu, Chunming, Zhang, Yuchi, Li, Sainan
Format: Article
Language:English
Subjects:
Carbon
Chromatography
Cytotoxicity
Dehydrogenase
Dehydrogenases
Dispersants
Experiments
High speed
Inhibitors
Inonotus obliquus
Ionic liquids
L-Lactate dehydrogenase
Lactate dehydrogenase
lactate dehydrogenase inhibitor
Lactic acid
Lanosterol
Ligands
Liquid chromatography
Lung cancer
Myocardial infarction
Reproducibility
Solvents
Triterpenoids
Ultrafiltration
ultrasonic-assisted extraction
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Summary:Extracts of the fungus exhibit cytotoxic properties against different cancers; hence, this fungal species has been extensively studied. This study aimed to extract total triterpenoids from using ionic liquids (ILs) and separate potential lactate dehydrogenase (LDH) inhibitors via ultrafiltration (UF)-high-speed countercurrent chromatography (HSCCC). Total triterpenoids from were extracted by performing a single-factor experiment and employing a central composite design via ultrasonic-assisted extraction (UAE) and heat-assisted extraction (HAE). The extract was composed of 1-butyl-3-methylimidazolium bromide as the IL and methanol as the dispersant. Ultrafiltration-liquid chromatography (UF-LC) was used to rapidly scan the LDH inhibitors and betulin and lanosterol were identified as potential inhibitors. To obtain these target compounds, betulin and lanosterol with the purities of 95.9% and 97.8% were isolated from HSCCC within 120 min. Their structures were identified using several techniques, among which IL-HAE was fast and effective. This study reports the extraction of triterpenoids from by IL for the first time. Collectively, the findings demonstrate that UF-LC is an effective tool for screening potential LDH inhibitors from crude extracts of and may help to identify bioactive substances against myocardial infarction, whereas high-purity compounds can be separated via UF-HSCCC.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules26092467