Alginate Gel Encapsulated with Enzybiotics Cocktail Is Effective against Multispecies Biofilms

The development of new and effective antibacterials for pharmaceutical or cosmetic skin care that have a low potential for the emergence and expansion of bacterial resistance is of high demand in scientific and applied research. Great hopes are placed on alternative agents such as bactericidal pepti...

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Bibliographic Details
Published in:Gels 2024-01, Vol.10 (1), p.60
Main Authors: Vasina, Daria V, Antonova, Nataliia P, Shidlovskaya, Elena V, Kuznetsova, Nadezhda A, Grishin, Alexander V, Akoulina, Elizaveta A, Trusova, Ekaterina A, Lendel, Anastasiya M, Mazunina, Elena P, Kozlova, Sofia R, Dudun, Andrei A, Bonartsev, Anton P, Lunin, Vladimir G, Gushchin, Vladimir A
Format: Article
Language:English
Subjects:
alginate gel
Alginates
Antibiotics
Antimicrobial agents
Bacteria
Bacterial pneumonia
Bacteriocins
Biofilms
Consortia
Cosmetics
Drug resistance
Drug therapy
endolysin
enzybiotics
Enzymes
Health aspects
Hydrolases
Klebsiella
lysostaphin
Medical research
Medicine, Experimental
Methicillin
Microorganisms
Pathogens
Pneumonia
polymicrobial infection
Polysaccharides
Skin
Staphylococcus infections
Tetracycline
Tetracyclines
Toiletries
Toiletries industry
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Summary:The development of new and effective antibacterials for pharmaceutical or cosmetic skin care that have a low potential for the emergence and expansion of bacterial resistance is of high demand in scientific and applied research. Great hopes are placed on alternative agents such as bactericidal peptidoglycan hydrolases, depolymerases, etc. Enzybiotic-based preparations are being studied for the treatment of various infections and, among others, can be used as topical formulations and dressings with protein-polysaccharide complexes. Here, we investigate the antibiofilm properties of a novel enzybiotic cocktail of phage endolysin LysSi3 and bacteriocin lysostaphin, formulated in the alginate gel matrix and its ability to control the opportunistic skin-colonizing bacteria , , and , as well as mixed-species biofilms. Our results propose that the application of SiL-gel affects different components of biofilm extracellular polymeric substances, disrupts the matrix, and eliminates the bacteria embedded in it. This composition is highly effective against biofilms composed of Gram-negative and Gram-positive species and does not possess significant cytotoxic effects. Our data form the basis for the development of antibacterial skin care products with a gentle but effective mode of action.
ISSN:2310-2861
2310-2861
DOI:10.3390/gels10010060