Application of Lectin Microarrays for Biomarker Discovery

Many proteins in living organisms are glycosylated. As their glycan patterns exhibit protein‐, cell‐, and tissue‐specific heterogeneity, changes in the glycosylation levels could serve as useful indicators of various pathological and physiological states. Thus, the identification of glycoprotein bio...

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Bibliographic Details
Published in:ChemistryOpen (Weinheim) 2020-03, Vol.9 (3), p.285-300
Main Authors: Dang, Kai, Zhang, Wenjuan, Jiang, Shanfeng, Lin, Xiao, Qian, Airong
Format: Article
Language:English
Subjects:
Biological properties
Biomarkers
Cancer
Cell adhesion & migration
Disease
Gene expression
Glycan
glycans
glycomics
Glycoproteins
glycoproteomics
Heterogeneity
Immunoglobulins
lectin microarrays
Lectins
Methods
Physiology
Proteins
Review
Reviews
Technology assessment
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Summary:Many proteins in living organisms are glycosylated. As their glycan patterns exhibit protein‐, cell‐, and tissue‐specific heterogeneity, changes in the glycosylation levels could serve as useful indicators of various pathological and physiological states. Thus, the identification of glycoprotein biomarkers from specific changes in the glycan profiles of glycoproteins is a trending field. Lectin microarrays provide a new glycan analysis platform, which enables rapid and sensitive analysis of complex glycans without requiring the release of glycans from the protein. Recent developments in lectin microarray technology enable high‐throughput analysis of glycans in complex biological samples. In this review, we will discuss the basic concepts and recent progress in lectin microarray technology, the application of lectin microarrays in biomarker discovery, and the challenges and future development of this technology. Given the tremendous technical advancements that have been made, lectin microarrays will become an indispensable tool for the discovery of glycoprotein biomarkers. A powerful tool to find novel biomarkers! Lectin microarray technology is a fast, high‐throughput, and inexpensive tool for the discovery of glycol‐biomarkers in complex biological samples. This review summarizes the recent progress and application of lectin microarrays in biomarker discovery under different physiopathological states.
ISSN:2191-1363
2191-1363
DOI:10.1002/open.201900326