Host/Malassezia Interaction: A Quantitative, Non-Invasive Method Profiling Oxylipin Production Associates Human Skin Eicosanoids with Malassezia

Malassezia are common components of human skin, and as the dominant human skin eukaryotic microbe, they take part in complex microbe–host interactions. Other phylogenetically related fungi (including within Ustilagomycotina) communicate with their plant host through bioactive oxygenated polyunsatura...

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Bibliographic Details
Published in:Metabolites 2021-10, Vol.11 (10), p.700
Main Authors: Ambaw, Yohannes Abere, Pagac, Martin P., Irudayaswamy, Antony S., Raida, Manfred, Bendt, Anne K., Torta, Federico T., Wenk, Markus R., Dawson, Thomas L.
Format: Article
Language:English
Subjects:
Adhesives
Communication
Cytochrome
Discriminant analysis
Eicosanoids
Fatty acids
Fungi
Host plants
Immune system
lipidomics
Lipids
Malassezia
Metabolic pathways
Metabolism
Metabolites
Microbiota
mycobiome
non-invasive
oxylipins
Pathogens
Phylogeny
Polyunsaturated fatty acids
Skin
Structure-function relationships
Virulence
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Summary:Malassezia are common components of human skin, and as the dominant human skin eukaryotic microbe, they take part in complex microbe–host interactions. Other phylogenetically related fungi (including within Ustilagomycotina) communicate with their plant host through bioactive oxygenated polyunsaturated fatty acids, generally known as oxylipins, by regulating the plant immune system to increase their virulence. Oxylipins are similar in structure and function to human eicosanoids, which modulate the human immune system. This study reports the development of a highly sensitive mass-spectrometry-based method to capture and quantify bioactive oxygenated polyunsaturated fatty acids from the human skin surface and in vitro Malassezia cultures. It confirms that Malassezia are capable of synthesizing eicosanoid-like lipid mediators in vitro in a species dependent manner, many of which are found on human skin. This method enables sensitive identification and quantification of bioactive lipid mediators from human skin that may be derived from metabolic pathways shared between skin and its microbial residents. This enables better cross-disciplinary and detailed studies to dissect the interaction between Malassezia and human skin, and to identify potential intervention points to promote or abrogate inflammation and to improve human skin health.
ISSN:2218-1989
2218-1989
DOI:10.3390/metabo11100700