Comparative study of environmental pollutants bisphenol A and bisphenol S on sexual differentiation of anteroventral periventricular nucleus and spermatogenesis

• Published in: Reproductive biology and endocrinology 2019-07, Vol.17 (1), p.53-53, Article 53
• Main Authors: John, Naham, Rehman, Humaira, Razak, Suhail, David, Mehwish, Ullah, Waheed, Afsar, Tayyaba, Almajwal, Ali, Alam, Iftikhar, Jahan, Sarwat
• Format: Article
• Language: English
• Subjects: 17β-Estradiol; Animals; Animals, Newborn; AVPV; Benzhydryl Compounds - pharmacology; Bisphenol A; Bisphenol S; Cancer; Development and progression; Developmental stages; Disorders of sex development; Dopamine receptors; Dopaminergic Neurons - drug effects; Dopaminergic Neurons - metabolism; Endocrine disruptor; Endocrine disruptors; Endocrine Disruptors - pharmacology; Environmental Pollutants - pharmacology; Estradiol - pharmacology; Estrogens - pharmacology; Female; Females; Gender differences; Health aspects; Hydroxylase; Hypothalamus; Hypothalamus, Anterior - drug effects; Hypothalamus, Anterior - pathology; Immunohistochemistry; Male; Neonates; Neurons; Olive oil; Periventricular nucleus; Phenols - pharmacology; Physiological aspects; Pollutants; Rats, Sprague-Dawley; Reproductive system; Risk factors; Rodents; Seminiferous tubule; Seminiferous Tubules - drug effects; Seminiferous Tubules - pathology; Sex differentiation; Sex Differentiation - drug effects; Sex Factors; Sexual dimorphism; Spermatogenesis; Spermatogenesis - drug effects; Sulfones - pharmacology; Testes; Testing; Toxicology; Tyrosine 3-monooxygenase
• ISSN: 1477-7827; 1477-7827
• DOI: 10.1186/s12958-019-0491-x

Bisphenol A is well known endocrine-disrupting chemical while Bisphenol S was considered a safe alternative. The present study aims to examine the comparative effects of xenobiotic bisphenol-A (BPA) and its substitute bisphenol-S (BPS) on spermatogenesis and development of sexually dimorphic nucleus population of dopaminergic neurons in the anteroventral periventricular nucleus (AVPV) of the hypothalamus in male pups. Sprague Dawley rat's pups were administered subcutaneously at the neonatal stage from postnatal day PND1 to PND 27. Thirty animals were divided into six experimental groups (6 animals/group). The first group served as control and was provided with normal olive oil. The four groups were treated with 2 μg/kg and 200 μg/kg of BPA and BPS, respectively. The sixth group was given with 50 μg/kg of estradiol dissolved in olive oil as a standard to find the development of dopaminergic tyrosine hydroxylase neurons in AVPV regions. Histological analysis for testicular tissues and immunohistochemistry for brain tissues was performed. The results revealed adverse histopathological changes in testis after administration of different doses of BPA and BPS. These degenerative changes were marked by highly significant (p